Please use this identifier to cite or link to this item: https://doi.org/10.1039/B915147H
Title: Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments
Authors: Zhang, Chi 
Zhao, Ziqing 
Rahim, Nur Aida Abdul
van Noort, Danny
Yu, Hanry 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Technology
Biochemical Research Methods
Chemistry, Multidisciplinary
Chemistry, Analytical
Nanoscience & Nanotechnology
Instruments & Instrumentation
Biochemistry & Molecular Biology
Chemistry
Science & Technology - Other Topics
MICROFLUIDIC CHANNELS
SPECIES-DIFFERENCES
HEPATOCYTE CULTURE
DRUG DISCOVERY
LUNG-CANCER
TOXICITY
SYSTEM
RAT
PHARMACOKINETICS
ENVIRONMENT
Issue Date: 1-Jan-2009
Publisher: ROYAL SOC CHEMISTRY
Citation: Zhang, Chi, Zhao, Ziqing, Rahim, Nur Aida Abdul, van Noort, Danny, Yu, Hanry (2009-01-01). Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments. LAB ON A CHIP 9 (22) : 3185-3192. ScholarBank@NUS Repository. https://doi.org/10.1039/B915147H
Abstract: We have developed a multi-channel 3D microfluidic cell culture system (multi-channel 3D-FCCS) with compartmentalized microenvironments for potential application in human drug screening. To this end, the multi-channel 3D-FCCS was designed for culturing different 3D cellular aggregates simultaneously to mimic multiple organs in the body. Four human cell types (C3A, A549, HK-2 and HPA) were chosen to represent the liver, lung, kidney and the adipose tissue, respectively. Cellular functions were optimized by supplementing the common medium with growth factors. However, TGF-β1 was found to enhance A549 functions but inhibit C3A functions. Therefore, TGF-β1 was specifically controlled-released inside the A549 compartment by means of gelatin microspheres mixed with cells, thus creating a cell-specific microenvironment. The function of A549 cells was enhanced while the functions of C3A, HK-2 and HPA cells were uncompromised, demonstrating the limited cross-talk between cell culture compartments similar to the in vivo situation. Such a multi-channel 3D-FCCS could be potentially used to supplement or even replace animal models in drug screening. © 2009 The Royal Society of Chemistry.
Source Title: LAB ON A CHIP
URI: https://scholarbank.nus.edu.sg/handle/10635/173665
ISSN: 14730197
14730189
DOI: 10.1039/B915147H
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