Please use this identifier to cite or link to this item:
https://doi.org/10.1039/B915147H
DC Field | Value | |
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dc.title | Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments | |
dc.contributor.author | Zhang, Chi | |
dc.contributor.author | Zhao, Ziqing | |
dc.contributor.author | Rahim, Nur Aida Abdul | |
dc.contributor.author | van Noort, Danny | |
dc.contributor.author | Yu, Hanry | |
dc.date.accessioned | 2020-08-31T07:17:37Z | |
dc.date.available | 2020-08-31T07:17:37Z | |
dc.date.issued | 2009-01-01 | |
dc.identifier.citation | Zhang, Chi, Zhao, Ziqing, Rahim, Nur Aida Abdul, van Noort, Danny, Yu, Hanry (2009-01-01). Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments. LAB ON A CHIP 9 (22) : 3185-3192. ScholarBank@NUS Repository. https://doi.org/10.1039/B915147H | |
dc.identifier.issn | 14730197 | |
dc.identifier.issn | 14730189 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/173665 | |
dc.description.abstract | We have developed a multi-channel 3D microfluidic cell culture system (multi-channel 3D-FCCS) with compartmentalized microenvironments for potential application in human drug screening. To this end, the multi-channel 3D-FCCS was designed for culturing different 3D cellular aggregates simultaneously to mimic multiple organs in the body. Four human cell types (C3A, A549, HK-2 and HPA) were chosen to represent the liver, lung, kidney and the adipose tissue, respectively. Cellular functions were optimized by supplementing the common medium with growth factors. However, TGF-β1 was found to enhance A549 functions but inhibit C3A functions. Therefore, TGF-β1 was specifically controlled-released inside the A549 compartment by means of gelatin microspheres mixed with cells, thus creating a cell-specific microenvironment. The function of A549 cells was enhanced while the functions of C3A, HK-2 and HPA cells were uncompromised, demonstrating the limited cross-talk between cell culture compartments similar to the in vivo situation. Such a multi-channel 3D-FCCS could be potentially used to supplement or even replace animal models in drug screening. © 2009 The Royal Society of Chemistry. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1039/b915147h | |
dc.language.iso | en | |
dc.publisher | ROYAL SOC CHEMISTRY | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Physical Sciences | |
dc.subject | Technology | |
dc.subject | Biochemical Research Methods | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Analytical | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Instruments & Instrumentation | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Chemistry | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | MICROFLUIDIC CHANNELS | |
dc.subject | SPECIES-DIFFERENCES | |
dc.subject | HEPATOCYTE CULTURE | |
dc.subject | DRUG DISCOVERY | |
dc.subject | LUNG-CANCER | |
dc.subject | TOXICITY | |
dc.subject | SYSTEM | |
dc.subject | RAT | |
dc.subject | PHARMACOKINETICS | |
dc.subject | ENVIRONMENT | |
dc.type | Article | |
dc.date.updated | 2020-07-19T09:05:06Z | |
dc.contributor.department | CHEMISTRY | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.1039/B915147H | |
dc.description.sourcetitle | LAB ON A CHIP | |
dc.description.volume | 9 | |
dc.description.issue | 22 | |
dc.description.page | 3185-3192 | |
dc.identifier.isiut | 000271243600002 | |
dc.description.place | UNITED KINGDOM | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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File | Description | Size | Format | Access Settings | Version | |
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2009_Lab Chip_Zhang.pdf | Published version | 1.07 MB | Adobe PDF | CLOSED | None |
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