Please use this identifier to cite or link to this item: https://doi.org/10.1039/B915147H
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dc.titleTowards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments
dc.contributor.authorZhang, Chi
dc.contributor.authorZhao, Ziqing
dc.contributor.authorRahim, Nur Aida Abdul
dc.contributor.authorvan Noort, Danny
dc.contributor.authorYu, Hanry
dc.date.accessioned2020-08-31T07:17:37Z
dc.date.available2020-08-31T07:17:37Z
dc.date.issued2009-01-01
dc.identifier.citationZhang, Chi, Zhao, Ziqing, Rahim, Nur Aida Abdul, van Noort, Danny, Yu, Hanry (2009-01-01). Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments. LAB ON A CHIP 9 (22) : 3185-3192. ScholarBank@NUS Repository. https://doi.org/10.1039/B915147H
dc.identifier.issn14730197
dc.identifier.issn14730189
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173665
dc.description.abstractWe have developed a multi-channel 3D microfluidic cell culture system (multi-channel 3D-FCCS) with compartmentalized microenvironments for potential application in human drug screening. To this end, the multi-channel 3D-FCCS was designed for culturing different 3D cellular aggregates simultaneously to mimic multiple organs in the body. Four human cell types (C3A, A549, HK-2 and HPA) were chosen to represent the liver, lung, kidney and the adipose tissue, respectively. Cellular functions were optimized by supplementing the common medium with growth factors. However, TGF-β1 was found to enhance A549 functions but inhibit C3A functions. Therefore, TGF-β1 was specifically controlled-released inside the A549 compartment by means of gelatin microspheres mixed with cells, thus creating a cell-specific microenvironment. The function of A549 cells was enhanced while the functions of C3A, HK-2 and HPA cells were uncompromised, demonstrating the limited cross-talk between cell culture compartments similar to the in vivo situation. Such a multi-channel 3D-FCCS could be potentially used to supplement or even replace animal models in drug screening. © 2009 The Royal Society of Chemistry.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1039/b915147h
dc.language.isoen
dc.publisherROYAL SOC CHEMISTRY
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysical Sciences
dc.subjectTechnology
dc.subjectBiochemical Research Methods
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry, Analytical
dc.subjectNanoscience & Nanotechnology
dc.subjectInstruments & Instrumentation
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry
dc.subjectScience & Technology - Other Topics
dc.subjectMICROFLUIDIC CHANNELS
dc.subjectSPECIES-DIFFERENCES
dc.subjectHEPATOCYTE CULTURE
dc.subjectDRUG DISCOVERY
dc.subjectLUNG-CANCER
dc.subjectTOXICITY
dc.subjectSYSTEM
dc.subjectRAT
dc.subjectPHARMACOKINETICS
dc.subjectENVIRONMENT
dc.typeArticle
dc.date.updated2020-07-19T09:05:06Z
dc.contributor.departmentDEPT OF CHEMISTRY
dc.contributor.departmentDEPT OF PHYSIOLOGY
dc.description.doi10.1039/B915147H
dc.description.sourcetitleLAB ON A CHIP
dc.description.volume9
dc.description.issue22
dc.description.page3185-3192
dc.identifier.isiut000271243600002
dc.description.placeUNITED KINGDOM
dc.published.statePublished
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