Please use this identifier to cite or link to this item:
https://doi.org/10.1096/fj.201900057R
Title: | Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism | Authors: | Yap, Jasmine Lye Yee Tai, Yee Kit Frohlich, Juerg Fong, Charlene Hui Hua Yin, Jocelyn Naixin Foo, Zi Ling Ramanan, Sharanya Beyer, Christian Toh, Shi Jie Casarosa, Marco Bharathy, Narendra Kala, Monica Palanichamy Egli, Marcel Taneja, Reshma Lee, Chuen Neng Franco-Obregon, Alfredo |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Biology Cell Biology Life Sciences & Biomedicine - Other Topics pulsed electromagnetic fields reactive oxygen species PGC-1 alpha calcineurin mitochondriogenesis MECHANOSENSITIVE ION CHANNELS MYOBLAST DIFFERENTIATION C2C12 MYOBLASTS DISTINCT ROLES MUSCLE TRPC1 PGC-1-ALPHA CALCIUM PROLIFERATION CALCINEURIN |
Issue Date: | 1-Nov-2019 | Publisher: | FEDERATION AMER SOC EXP BIOL | Citation: | Yap, Jasmine Lye Yee, Tai, Yee Kit, Frohlich, Juerg, Fong, Charlene Hui Hua, Yin, Jocelyn Naixin, Foo, Zi Ling, Ramanan, Sharanya, Beyer, Christian, Toh, Shi Jie, Casarosa, Marco, Bharathy, Narendra, Kala, Monica Palanichamy, Egli, Marcel, Taneja, Reshma, Lee, Chuen Neng, Franco-Obregon, Alfredo (2019-11-01). Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB JOURNAL 33 (11) : 12853-12872. ScholarBank@NUS Repository. https://doi.org/10.1096/fj.201900057R | Abstract: | © FASEB We show that both supplemental and ambient magnetic fields modulate myogenesis. A lone 10 min exposure of myoblasts to 1.5 mT amplitude supplemental pulsed magnetic fields (PEMFs) accentuated in vitro myogenesis by stimulating transient receptor potential (TRP)-C1-mediated calcium entry and downstream nuclear factor of activated T cells (NFAT)-transcriptional and P300/CBP-associated factor (PCAF)-epigenetic cascades, whereas depriving myoblasts of ambient magnetic fields slowed myogenesis, reduced TRPC1 expression, and silenced NFAT-transcriptional and PCAF-epigenetic cascades. The expression levels of peroxisome proliferatoractivated receptor g coactivator 1α, the master regulator of mitochondriogenesis, was also enhanced by brief PEMF exposure. Accordingly, mitochondriogenesis and respiratory capacity were both enhanced with PEMF exposure, paralleling TRPC1 expression and pharmacological sensitivity. Clustered regularly interspaced short palindromic repeats-Cas9 knockdown of TRPC1 precluded proliferative and mitochondrial responses to supplemental PEMFs, whereas small interfering RNA gene silencing of TRPM7 did not, coinciding with data that magnetoreception did not coincide with the expression or function of other TRP channels. The aminoglycoside antibiotics antagonized and down-regulated TRPC1 expression and, when applied concomitantly with PEMF exposure, attenuated PEMF-stimulated calcium entry, mitochondrial respiration, proliferation, differentiation, and epigenetic directive in myoblasts, elucidating why the developmental potential of magnetic fields may have previously escaped detection. Mitochondrial-based survival adaptations were also activated upon PEMF stimulation. Magnetism thus deploys an authentic myogenic directive that relies on an interplay between mitochondria and TRPC1 to reach fruition.—Yap, J. L. Y., Tai, Y. K., Fröhlich, J., Fong, C. H. H., Yin, J. N., Foo, Z. L., Ramanan, S., Beyer, C., Toh, S. J., Casarosa, M., Bharathy, N., Kala, M. P., Egli, M., Taneja, R., Lee, C. N., Franco-Obregón, A. Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB J. 33, 12853–12872 (2019). www.fasebj.org. | Source Title: | FASEB JOURNAL | URI: | https://scholarbank.nus.edu.sg/handle/10635/173636 | ISSN: | 08926638 15306860 |
DOI: | 10.1096/fj.201900057R |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Yap et al. 2019.pdf | 3.44 MB | Adobe PDF | CLOSED | None |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.