Please use this identifier to cite or link to this item: https://doi.org/10.1096/fj.201900057R
Title: Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism
Authors: Yap, Jasmine Lye Yee
Tai, Yee Kit 
Frohlich, Juerg
Fong, Charlene Hui Hua
Yin, Jocelyn Naixin
Foo, Zi Ling 
Ramanan, Sharanya
Beyer, Christian
Toh, Shi Jie 
Casarosa, Marco
Bharathy, Narendra
Kala, Monica Palanichamy
Egli, Marcel 
Taneja, Reshma 
Lee, Chuen Neng 
Franco-Obregon, Alfredo 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biology
Cell Biology
Life Sciences & Biomedicine - Other Topics
pulsed electromagnetic fields
reactive oxygen species
PGC-1 alpha
calcineurin
mitochondriogenesis
MECHANOSENSITIVE ION CHANNELS
MYOBLAST DIFFERENTIATION
C2C12 MYOBLASTS
DISTINCT ROLES
MUSCLE
TRPC1
PGC-1-ALPHA
CALCIUM
PROLIFERATION
CALCINEURIN
Issue Date: 1-Nov-2019
Publisher: FEDERATION AMER SOC EXP BIOL
Citation: Yap, Jasmine Lye Yee, Tai, Yee Kit, Frohlich, Juerg, Fong, Charlene Hui Hua, Yin, Jocelyn Naixin, Foo, Zi Ling, Ramanan, Sharanya, Beyer, Christian, Toh, Shi Jie, Casarosa, Marco, Bharathy, Narendra, Kala, Monica Palanichamy, Egli, Marcel, Taneja, Reshma, Lee, Chuen Neng, Franco-Obregon, Alfredo (2019-11-01). Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB JOURNAL 33 (11) : 12853-12872. ScholarBank@NUS Repository. https://doi.org/10.1096/fj.201900057R
Abstract: © FASEB We show that both supplemental and ambient magnetic fields modulate myogenesis. A lone 10 min exposure of myoblasts to 1.5 mT amplitude supplemental pulsed magnetic fields (PEMFs) accentuated in vitro myogenesis by stimulating transient receptor potential (TRP)-C1-mediated calcium entry and downstream nuclear factor of activated T cells (NFAT)-transcriptional and P300/CBP-associated factor (PCAF)-epigenetic cascades, whereas depriving myoblasts of ambient magnetic fields slowed myogenesis, reduced TRPC1 expression, and silenced NFAT-transcriptional and PCAF-epigenetic cascades. The expression levels of peroxisome proliferatoractivated receptor g coactivator 1α, the master regulator of mitochondriogenesis, was also enhanced by brief PEMF exposure. Accordingly, mitochondriogenesis and respiratory capacity were both enhanced with PEMF exposure, paralleling TRPC1 expression and pharmacological sensitivity. Clustered regularly interspaced short palindromic repeats-Cas9 knockdown of TRPC1 precluded proliferative and mitochondrial responses to supplemental PEMFs, whereas small interfering RNA gene silencing of TRPM7 did not, coinciding with data that magnetoreception did not coincide with the expression or function of other TRP channels. The aminoglycoside antibiotics antagonized and down-regulated TRPC1 expression and, when applied concomitantly with PEMF exposure, attenuated PEMF-stimulated calcium entry, mitochondrial respiration, proliferation, differentiation, and epigenetic directive in myoblasts, elucidating why the developmental potential of magnetic fields may have previously escaped detection. Mitochondrial-based survival adaptations were also activated upon PEMF stimulation. Magnetism thus deploys an authentic myogenic directive that relies on an interplay between mitochondria and TRPC1 to reach fruition.—Yap, J. L. Y., Tai, Y. K., Fröhlich, J., Fong, C. H. H., Yin, J. N., Foo, Z. L., Ramanan, S., Beyer, C., Toh, S. J., Casarosa, M., Bharathy, N., Kala, M. P., Egli, M., Taneja, R., Lee, C. N., Franco-Obregón, A. Ambient and supplemental magnetic fields promote myogenesis via a TRPC1-mitochondrial axis: evidence of a magnetic mitohormetic mechanism. FASEB J. 33, 12853–12872 (2019). www.fasebj.org.
Source Title: FASEB JOURNAL
URI: https://scholarbank.nus.edu.sg/handle/10635/173636
ISSN: 08926638
15306860
DOI: 10.1096/fj.201900057R
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