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https://doi.org/10.1007/978-1-4939-7592-1_11
| Title: | Sphingolipid Analysis in Clinical Research | Authors: | Burla, B Muralidharan, S Wenk, MR Torta, F |
Keywords: | Ceramide Clinical mass spectrometry Glucosylceramide Lipidomics Mass spectrometry Quality control Sphingolipidomics Sphingolipids Sphingomyelin Sphingosine-1-phosphate Blood Specimen Collection Chromatography, Liquid Humans Mass Spectrometry Metabolomics Research Design Sphingolipids |
Issue Date: | 1-Jan-2018 | Publisher: | Springer New York | Citation: | Burla, B, Muralidharan, S, Wenk, MR, Torta, F (2018-01-01). Sphingolipid Analysis in Clinical Research. Methods Mol Biol 1730 : 135-162. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-4939-7592-1_11 | Abstract: | © Springer Science+Business Media, LLC 2018. Sphingolipids are the most diverse class of lipids due to the numerous variations in their structural components. This diversity is also reflected in their extremely different functions. Sphingolipids are not only constituents of cell membranes but have also emerged as key signaling molecules involved in a variety of cellular functions, such as cell growth and differentiation, proliferation, and apoptotic cell death. Lipidomic analyses in clinical research have identified pathways and products of sphingolipid metabolism that are altered in several human pathologies. In this article, we describe how to properly design a lipidomic experiment in clinical research, how to handle plasma and serum samples for this purpose, and how to measure sphingolipids using liquid chromatography-mass spectrometry. | Source Title: | Methods Mol Biol | URI: | https://scholarbank.nus.edu.sg/handle/10635/173274 | ISSN: | 10643745 19406029 |
DOI: | 10.1007/978-1-4939-7592-1_11 |
| Appears in Collections: | Staff Publications Elements |
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| 65. Sphingolipid Analysis in Clinical Research.pdf | Published version | 286.81 kB | Adobe PDF | CLOSED | None |
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