Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.19042
Title: Defining key roles for auxiliary proteins in an ABC transporter that maintains bacterial outer membrane lipid asymmetry
Authors: Thong, Shuhua 
Ercan, Bilge 
Torte, Federico 
Fong, Zhen Yang 
Wong, Hui Yi Alvina
Wenk, Markus R 
Chng, Shu-Sin 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
ESCHERICHIA-COLI
SALMONELLA-TYPHIMURIUM
SULFATE TRANSPORTER
BINDING PROTEIN
STAS DOMAIN
LIPOPOLYSACCHARIDE
COMPLEX
BIOGENESIS
PHOSPHOLIPIDS
MECHANISMS
Issue Date: 2016
Publisher: ELIFE SCIENCES PUBLICATIONS LTD
Citation: Thong, Shuhua, Ercan, Bilge, Torte, Federico, Fong, Zhen Yang, Wong, Hui Yi Alvina, Wenk, Markus R, Chng, Shu-Sin (2016). Defining key roles for auxiliary proteins in an ABC transporter that maintains bacterial outer membrane lipid asymmetry. ELIFE 5 (AUGUST). ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.19042
Abstract: © Thong et al. In Gram-negative bacteria, lipid asymmetry is critical for the function of the outer membrane (OM) as a selective permeability barrier, but how it is established and maintained is poorly understood. Here, we characterize a non-canonical ATP-binding cassette (ABC) transporter in Escherichia coli that provides energy for maintaining OM lipid asymmetry via the transport of aberrantly localized phospholipids (PLs) from the OM to the inner membrane (IM). We establish that the transporter comprises canonical components, MlaF and MlaE, and auxiliary proteins, MlaD and MlaB, of previously unknown functions. We further demonstrate that MlaD forms extremely stable hexamers within the complex, functions in substrate binding with strong affinity for PLs, and modulates ATP hydrolytic activity. In addition, MlaB plays critical roles in both the assembly and activity of the transporter. Our work provides mechanistic insights into how the MlaFEDB complex participates in ensuring active retrograde PL transport to maintain OM lipid asymmetry.
Source Title: ELIFE
URI: https://scholarbank.nus.edu.sg/handle/10635/170492
ISSN: 2050084X
2050084X
DOI: 10.7554/eLife.19042
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