Please use this identifier to cite or link to this item: https://doi.org/10.1002/anie.201503640
Title: A Photoactivatable AIE Polymer for Light-Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking
Authors: YUAN YOUYONG 
Zhang Chongjing 
LIU BIN 
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
aggregation-induced emission
endo/lysosomes
gene delivery
polymers
reactive oxygen species
AGGREGATION-INDUCED-EMISSION
BIOMEDICAL APPLICATIONS
CANCER-CELLS
THERAPY
POLYETHYLENIMINE
EFFICIENT
PEPTIDE
VECTOR
BIOPROBE
Issue Date: 21-Sep-2015
Publisher: Wiley-VCH
Citation: YUAN YOUYONG, Zhang Chongjing, LIU BIN (2015-09-21). A Photoactivatable AIE Polymer for Light-Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking. Angewandte Chemie - International Edition 54 (39) : 11419-11423. ScholarBank@NUS Repository. https://doi.org/10.1002/anie.201503640
Abstract: Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation-induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self-assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light-controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process. Special delivery! A photoactive polymeric gene delivery vector can concurrently escape from the endo/lysosome and unpack nucleic acids in cytosol in a single light irradiation process. The polymer, which comprises a fluorogen with aggregation-enhanced-emission characteristics and a linker that can be cleaved by reactive oxygen species, forms nanoparticles that bind DNA and are endocytosed by cells.
Source Title: Angewandte Chemie - International Edition
URI: https://scholarbank.nus.edu.sg/handle/10635/169647
ISSN: 1433-7851
1521-3773
DOI: 10.1002/anie.201503640
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