Please use this identifier to cite or link to this item: https://doi.org/10.1002/anie.201503640
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dc.titleA Photoactivatable AIE Polymer for Light-Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking
dc.contributor.authorYUAN YOUYONG
dc.contributor.authorZhang Chongjing
dc.contributor.authorLIU BIN
dc.date.accessioned2020-06-11T02:14:36Z
dc.date.available2020-06-11T02:14:36Z
dc.date.issued2015-09-21
dc.identifier.citationYUAN YOUYONG, Zhang Chongjing, LIU BIN (2015-09-21). A Photoactivatable AIE Polymer for Light-Controlled Gene Delivery: Concurrent Endo/Lysosomal Escape and DNA Unpacking. Angewandte Chemie - International Edition 54 (39) : 11419-11423. ScholarBank@NUS Repository. https://doi.org/10.1002/anie.201503640
dc.identifier.issn1433-7851
dc.identifier.issn1521-3773
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/169647
dc.description.abstractEndo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation-induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self-assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light-controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process. Special delivery! A photoactive polymeric gene delivery vector can concurrently escape from the endo/lysosome and unpack nucleic acids in cytosol in a single light irradiation process. The polymer, which comprises a fluorogen with aggregation-enhanced-emission characteristics and a linker that can be cleaved by reactive oxygen species, forms nanoparticles that bind DNA and are endocytosed by cells.
dc.language.isoen
dc.publisherWiley-VCH
dc.sourceElements
dc.subjectScience & Technology
dc.subjectPhysical Sciences
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry
dc.subjectaggregation-induced emission
dc.subjectendo/lysosomes
dc.subjectgene delivery
dc.subjectpolymers
dc.subjectreactive oxygen species
dc.subjectAGGREGATION-INDUCED-EMISSION
dc.subjectBIOMEDICAL APPLICATIONS
dc.subjectCANCER-CELLS
dc.subjectTHERAPY
dc.subjectPOLYETHYLENIMINE
dc.subjectEFFICIENT
dc.subjectPEPTIDE
dc.subjectVECTOR
dc.subjectBIOPROBE
dc.typeArticle
dc.date.updated2020-06-10T08:17:22Z
dc.contributor.departmentDEPT OF CHEMICAL & BIOMOLECULAR ENGG
dc.description.doi10.1002/anie.201503640
dc.description.sourcetitleAngewandte Chemie - International Edition
dc.description.volume54
dc.description.issue39
dc.description.page11419-11423
dc.published.statePublished
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