Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/168648
Title: Temperature and Mg2+ Sensing by a Novel PhoP-PhoQ Two-component System for Regulation of Virulence in Edwardsiella tarda
Authors: Chakraborty, Smarajit 
Li, Mo
Chatterjee, Chiradip 
Sivaraman, J 
Leung, Ka Yin
Mok, Yu-Keung 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
COMPLETE GENOME SEQUENCE
ESCHERICHIA-COLI
SENSOR DOMAIN
MOLECULAR CHARACTERIZATION
SECRETION SYSTEM
SIGNAL-DETECTION
GENE-EXPRESSION
SALMONELLA
IDENTIFICATION
PATHOGENESIS
Issue Date: 1-Dec-2010
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation: Chakraborty, Smarajit, Li, Mo, Chatterjee, Chiradip, Sivaraman, J, Leung, Ka Yin, Mok, Yu-Keung (2010-12-01). Temperature and Mg2+ Sensing by a Novel PhoP-PhoQ Two-component System for Regulation of Virulence in Edwardsiella tarda. JOURNAL OF BIOLOGICAL CHEMISTRY 285 (50) : 38876-38888. ScholarBank@NUS Repository.
Abstract: The PhoP-PhoQ two-component system is commonly used by bacteria to sense environmental factors. Here we show that the PhoP-PhoQ system of Edwardsiella tarda detects changes in environmental temperature and Mg2+ concentration as well as regulates the type III and VI secretion systems through direct activation of esrB. Protein secretion is activated from 23 to 35°C or at low Mg2+ concentrations, but it is suppressed at or below 20°C, at or above 37°C, or at high Mg2+ concentrations. The effects of temperature and Mg2+ concentration are additive. The PhoQ sensor domain has a low Tm of 37.9°C, and it detects temperatures through a conformational change of its secondary structure. Mutation of specific Pro or Thr residues increased the stability of the PhoQ sensor drastically, altering its temperature-sensing ability. The PhoQ sensor detects Mg2+ concentration through the direct binding of Mg2+ to a cluster of acidic residues (DDDSAD) and through changes that likely affect its tertiary structure. Here, we describe for the first time the use of PhoP-PhoQ as a temperature sensor for bacterial virulence control. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Source Title: JOURNAL OF BIOLOGICAL CHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/168648
ISSN: 00219258
1083351X
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