Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0022507
Title: Biocompatibility and biodegradation studies of subconjunctival implants in rabbit eyes
Authors: Peng Y.
Ang M. 
Foo S.
Lee W.S.
Ma Z.
Venkatraman S.S.
Wong T.T. 
Keywords: biopolymer
dexamethasone plus tobramycin
polycaprolactone
polyglactin
drug carrier
lactic acid
lactide caprolactone copolymer
lactide-caprolactone copolymer
polyester
polyglycolic acid
polylactic acid polyglycolic acid copolymer
polylactic acid-polyglycolic acid copolymer
water
animal experiment
animal tissue
anterior eye segment
article
biocompatibility
biodegradation
chemosis
conjunctiva
conjunctival hyperemia
controlled study
drug delivery system
enucleation
feasibility study
histopathology
immunofluorescence
immunohistochemistry
implant
in vitro study
in vivo study
inflammation
microfilm
molecular weight
nonhuman
optical coherence tomography
rabbit
slit lamp
subconjunctival implant
vascularization
viscosity
water absorption
weight reduction
absorption
animal
chemistry
conjunctiva
cytology
drug effect
materials testing
metabolism
prostheses and orthoses
Oryctolagus cuniculus
Absorption
Animals
Conjunctiva
Drug Carriers
Feasibility Studies
Lactic Acid
Materials Testing
Molecular Weight
Polyesters
Polyglycolic Acid
Prostheses and Implants
Rabbits
Water
Issue Date: 2011
Citation: Peng Y., Ang M., Foo S., Lee W.S., Ma Z., Venkatraman S.S., Wong T.T. (2011). Biocompatibility and biodegradation studies of subconjunctival implants in rabbit eyes. PLoS ONE 6 (7) : e22507. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0022507
Abstract: Sustained ocular drug delivery is difficult to achieve. Most drugs have poor penetration due to the multiple physiological barriers of the eye and are rapidly cleared if applied topically. Biodegradable subconjunctival implants with controlled drug release may circumvent these two problems. In our study, two microfilms (poly [d,l-lactide-co-glycolide] PLGA and poly[d,l-lactide-co-caprolactone] PLC were developed and evaluated for their degradation behavior in vitro and in vivo. We also evaluated the biocompatibility of both microfilms. Eighteen eyes (9 rabbits) were surgically implanted with one type of microfilm in each eye. Serial anterior-segment optical coherence tomography (AS-OCT) scans together with serial slit-lamp microscopy allowed us to measure thickness and cross-sectional area of the microfilms. In vitro studies revealed bulk degradation kinetics for both microfilms, while in vivo studies demonstrated surface erosion kinetics. Serial slit-lamp microscopy revealed no significant inflammation or vascularization in both types of implants (mean increase in vascularity grade PLGA50/50 12±0.5% vs. PLC70/30 15±0.6%; P = 0.91) over a period of 6 months. Histology, immunohistochemistry and immuno-fluorescence also revealed no significant inflammatory reaction from either of the microfilms, which confirmed that both microfilms are biocompatible. The duration of the drug delivery can be tailored by selecting the materials, which have different degradation kinetics, to suit the desired clinical therapeutic application. © 2011 Peng et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161792
ISSN: 19326203
DOI: 10.1371/journal.pone.0022507
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