Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0058578
Title: A Unifying Mechanism for Cancer Cell Death through Ion Channel Activation by HAMLET
Authors: Storm P.
Kjaer Klausen T.
Trulsson M.
Ho CS J.
Dosnon M.
Westergren T.
Chao Y. 
Rydström A.
Yang H. 
Pedersen S.F.
Svanborg C.
Keywords: antineoplastic agent
calcium ion
human alpha lactalbumin made lethal to tumor cell
mitogen activated protein kinase p38
potassium ion
sodium ion
unclassified drug
antineoplastic activity
apoptosis
article
cell structure
cell viability
controlled study
enzyme activation
enzyme inhibition
enzyme phosphorylation
human
human cell
immunostimulation
ion transport
nucleotide sequence
signal transduction
tumor cell destruction
Biological Transport
Calcium
Cell Death
Cell Line, Tumor
Cluster Analysis
Gene Expression Profiling
Humans
Immunity, Innate
Intracellular Space
Ion Channels
Lactalbumin
Oleic Acids
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Potassium
Signal Transduction
Sodium
Issue Date: 2013
Citation: Storm P., Kjaer Klausen T., Trulsson M., Ho CS J., Dosnon M., Westergren T., Chao Y., Rydström A., Yang H., Pedersen S.F., Svanborg C. (2013). A Unifying Mechanism for Cancer Cell Death through Ion Channel Activation by HAMLET. PLoS ONE 8 (3) : e58578. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0058578
Abstract: Ion channels and ion fluxes control many aspects of tissue homeostasis. During oncogenic transformation, critical ion channel functions may be perturbed but conserved tumor specific ion fluxes remain to be defined. Here we used the tumoricidal protein-lipid complex HAMLET as a probe to identify ion fluxes involved in tumor cell death. We show that HAMLET activates a non-selective cation current, which reached a magnitude of 2.74±0.88 nA within 1.43±0.13 min from HAMLET application. Rapid ion fluxes were essential for HAMLET-induced carcinoma cell death as inhibitors (amiloride, BaCl2), preventing the changes in free cellular Na+ and K+ concentrations also prevented essential steps accompanying carcinoma cell death, including changes in morphology, uptake, global transcription, and MAP kinase activation. Through global transcriptional analysis and phosphorylation arrays, a strong ion flux dependent p38 MAPK response was detected and inhibition of p38 signaling delayed HAMLET-induced death. Healthy, differentiated cells were resistant to HAMLET challenge, which was accompanied by innate immunity rather than p38-activation. The results suggest, for the first time, a unifying mechanism for the initiation of HAMLET's broad and rapid lethal effect on tumor cells. These findings are particularly significant in view of HAMLET's documented therapeutic efficacy in human studies and animal models. The results also suggest that HAMLET offers a two-tiered therapeutic approach, killing cancer cells while stimulating an innate immune response in surrounding healthy tissues. © 2013 Storm et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161337
ISSN: 19326203
DOI: 10.1371/journal.pone.0058578
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