Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbio.201800459
Title: Three-dimensional cellular imaging in thick biological tissue with confocal detection of one-photon fluorescence in the near-infrared II window
Authors: Wang, M
Chen, N 
Keywords: NIR-II
confocal
penetration depth
resolution
scattering
Issue Date: 1-Jul-2019
Publisher: Wiley
Citation: Wang, M, Chen, N (2019-07-01). Three-dimensional cellular imaging in thick biological tissue with confocal detection of one-photon fluorescence in the near-infrared II window. Journal of Biophotonics 12 (7) : e201800459-. ScholarBank@NUS Repository. https://doi.org/10.1002/jbio.201800459
Related Datasets: 10635/157286
Abstract: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Fluorescence imaging in the second near-infrared optical window (NIR-II, 900-1700 nm) has become a technique of choice for noninvasive in vivo imaging in recent years. Greater penetration depths with high spatial resolution and low background can be achieved with this NIR-II window, owing to low autofluorescence within this optical range and reduced scattering of long wavelength photons. Here, we present a novel design of confocal laser scanning microscope tailored for imaging in the NIR-II window. We showcase the outstanding penetration depth of our confocal setup with a series of imaging experiments. HeLa cells labeled with PbS quantum dots with a peak emission wavelength of 1276 nm can be visualized through a 3.5-mm-thick layer of scattering medium, which is a 0.8% Lipofundin solution. A commercially available organic dye IR-1061 (emission peak at 1132 nm), in its native form, is used for the first time, as a NIR-II fluorescence label in cellular imaging. Our confocal setup is capable of capturing optically sectioned images of IR-1061 labeled chondrocytes in fixed animal cartilage at a depth up to 800 μm, with a superb spatial resolution of around 2 μm.
Source Title: Journal of Biophotonics
URI: https://scholarbank.nus.edu.sg/handle/10635/157226
ISSN: 1864-063X
1864-0648
DOI: 10.1002/jbio.201800459
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