Please use this identifier to cite or link to this item: https://doi.org/10.25540/DAGE-D1B7
Title: Data from: Three-dimensional cellular imaging in thick biological tissue with confocal detection of one-photon fluorescence in the near-infrared II window
Creators: Wang, Menghan
Chen, Nanguang 
NUS Contact: CHEN NANGUANG
Subject: NIR-II
confocal
penetration depth
resolution
scattering
DOI: doi:10.25540/DAGE-D1B7
Description: 

Fluorescence imaging in the second near-infrared optical window (NIR-II, 900-1700 nm) has become a technique of choice for noninvasive in vivo imaging in recent years. Greater penetration depths with high spatial resolution and low background can be achieved with this NIR-II window, owing to low autofluorescence within this optical range and reduced scattering of long wavelength photons. Here, we present a novel design of confocal laser scanning microscope tailored for imaging in the NIR-II window. We showcase the outstanding penetration depth of our confocal setup with a series of imaging experiments. HeLa cells labeled with PbS quantum dots with a peak emission wavelength of 1276 nm can be visualized through a 3.5-mm-thick layer of scattering medium, which is a 0.8% Lipofundin solution. A commercially available organic dye IR-1061 (emission peak at 1132 nm), in its native form, is used for the first time, as a NIR-II fluorescence label in cellular imaging. Our confocal setup is capable of capturing optically sectioned images of IR-1061 labeled chondrocytes in fixed animal cartilage at a depth up to 800 μm, with a superb spatial resolution of around 2 μm.

Related Publications: 10635/157226
Citation: When using this data, please cite the original publication and also the dataset.
  • Wang, M., & Chen, N. (2019). Three‐dimensional cellular imaging in thick biological tissue with confocal detection of one‐photon fluorescence in the near‐infrared II window. Journal of biophotonics, e201800459.
  • Wang, Menghan, Chen, Nanguang (2019-07-30). Data from: Three-dimensional cellular imaging in thick biological tissue with confocal detection of one-photon fluorescence in the near-infrared II window. ScholarBank@NUS Repository. [Dataset]. https://doi.org/10.25540/DAGE-D1B7
License: Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
Appears in Collections:Staff Dataset

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