Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms4276
Title: A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson's disease models
Authors: Li L.
Zhang C.-W.
Chen G.Y. 
Zhu B. 
Chai C. 
Xu Q.-H. 
Tan E.-K. 
Zhu Q. 
Lim K.-L. 
Yao S.Q. 
Issue Date: 2014
Publisher: Springer Nature
Citation: Li L., Zhang C.-W., Chen G.Y., Zhu B., Chai C., Xu Q.-H., Tan E.-K., Zhu Q., Lim K.-L., Yao S.Q. (2014). A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson's disease models. Nature communications 5 : 1-10. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms4276
Abstract: The unusually high MAO-B activity consistently observed in Parkinson's disease (PD) patients has been proposed as a biomarker; however, this has not been realized due to the lack of probes suitable for MAO-B-specific detection in live cells/tissues. Here we report the first two-photon, small molecule fluorogenic probe (U1) that enables highly sensitive/specific and real-time imaging of endogenous MAO-B activities across biological samples. We also used U1 to confirm the reported inverse relationship between parkin and MAO-B in PD models. With no apparent toxicity, U1 may be used to monitor MAO-B activities in small animals during disease development. In clinical samples, we find elevated MAO-B activities only in B lymphocytes (not in fibroblasts), hinting that MAO-B activity in peripheral blood cells might be an accessible biomarker for rapid detection of PD. Our results provide important starting points for using small molecule imaging techniques to explore MAO-B at the organism level.
Source Title: Nature communications
URI: http://scholarbank.nus.edu.sg/handle/10635/150094
ISSN: 20411723
DOI: 10.1038/ncomms4276
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