Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms4276
DC FieldValue
dc.titleA sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson's disease models
dc.contributor.authorLi L.
dc.contributor.authorZhang C.-W.
dc.contributor.authorChen G.Y.
dc.contributor.authorZhu B.
dc.contributor.authorChai C.
dc.contributor.authorXu Q.-H.
dc.contributor.authorTan E.-K.
dc.contributor.authorZhu Q.
dc.contributor.authorLim K.-L.
dc.contributor.authorYao S.Q.
dc.date.accessioned2018-12-20T09:19:08Z
dc.date.available2018-12-20T09:19:08Z
dc.date.issued2014
dc.identifier.citationLi L., Zhang C.-W., Chen G.Y., Zhu B., Chai C., Xu Q.-H., Tan E.-K., Zhu Q., Lim K.-L., Yao S.Q. (2014). A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson's disease models. Nature communications 5 : 1-10. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms4276
dc.identifier.issn20411723
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/150094
dc.description.abstractThe unusually high MAO-B activity consistently observed in Parkinson's disease (PD) patients has been proposed as a biomarker; however, this has not been realized due to the lack of probes suitable for MAO-B-specific detection in live cells/tissues. Here we report the first two-photon, small molecule fluorogenic probe (U1) that enables highly sensitive/specific and real-time imaging of endogenous MAO-B activities across biological samples. We also used U1 to confirm the reported inverse relationship between parkin and MAO-B in PD models. With no apparent toxicity, U1 may be used to monitor MAO-B activities in small animals during disease development. In clinical samples, we find elevated MAO-B activities only in B lymphocytes (not in fibroblasts), hinting that MAO-B activity in peripheral blood cells might be an accessible biomarker for rapid detection of PD. Our results provide important starting points for using small molecule imaging techniques to explore MAO-B at the organism level.
dc.publisherSpringer Nature
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDEPT OF CHEMISTRY
dc.contributor.departmentDEPT OF PHYSIOLOGY
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1038/ncomms4276
dc.description.sourcetitleNature communications
dc.description.volume5
dc.description.page1-10
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
ncomms4276.pdf836.23 kBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

97
checked on May 17, 2019

WEB OF SCIENCETM
Citations

76
checked on Dec 24, 2018

Page view(s)

45
checked on May 13, 2019

Download(s)

4
checked on May 13, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.