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https://scholarbank.nus.edu.sg/handle/10635/133726
| Title: | The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos | Authors: | Vasuthevan, S. Ng, S.C. Edirisinghe, R. Bongso, A. Ratnam, S. |
Keywords: | buffer systems medium Mouse embryos ultrarapid freezing |
Issue Date: | 1992 | Citation: | Vasuthevan, S., Ng, S.C., Edirisinghe, R., Bongso, A., Ratnam, S. (1992). The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos. Fertility and Sterility 58 (6) : 1250-1253. ScholarBank@NUS Repository. | Abstract: | Bicarbonate-buffered HTF medium, Medicult, and T6 are as effective as PB1 medium when used in combination with DMSO in ultrarapid freezing of two-cell mouse embryos. However, the use of phosphate-buffered T6 results in reduced in vitro development and inner cell mass size as compared with bicarbonate- and Hepes-buffered T6 when used with 3.5 M of DMSO. Hence, the use of this media for ultrarapid freezing should be avoided when this concentration of DMSO is used. | Source Title: | Fertility and Sterility | URI: | http://scholarbank.nus.edu.sg/handle/10635/133726 | ISSN: | 00150282 |
| Appears in Collections: | Staff Publications |
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