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Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/133726
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dc.titleThe evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos
dc.contributor.authorVasuthevan, S.
dc.contributor.authorNg, S.C.
dc.contributor.authorEdirisinghe, R.
dc.contributor.authorBongso, A.
dc.contributor.authorRatnam, S.
dc.date.accessioned2016-12-20T08:39:21Z
dc.date.available2016-12-20T08:39:21Z
dc.date.issued1992
dc.identifier.citationVasuthevan, S., Ng, S.C., Edirisinghe, R., Bongso, A., Ratnam, S. (1992). The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos. Fertility and Sterility 58 (6) : 1250-1253. ScholarBank@NUS Repository.
dc.identifier.issn00150282
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/133726
dc.description.abstractBicarbonate-buffered HTF medium, Medicult, and T6 are as effective as PB1 medium when used in combination with DMSO in ultrarapid freezing of two-cell mouse embryos. However, the use of phosphate-buffered T6 results in reduced in vitro development and inner cell mass size as compared with bicarbonate- and Hepes-buffered T6 when used with 3.5 M of DMSO. Hence, the use of this media for ultrarapid freezing should be avoided when this concentration of DMSO is used.
dc.subjectbuffer systems
dc.subjectmedium
dc.subjectMouse embryos
dc.subjectultrarapid freezing
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.sourcetitleFertility and Sterility
dc.description.volume58
dc.description.issue6
dc.description.page1250-1253
dc.description.codenFESTA
dc.identifier.isiutNOT_IN_WOS
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