Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/133726
Title: The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos
Authors: Vasuthevan, S. 
Ng, S.C. 
Edirisinghe, R. 
Bongso, A. 
Ratnam, S. 
Keywords: buffer systems
medium
Mouse embryos
ultrarapid freezing
Issue Date: 1992
Source: Vasuthevan, S., Ng, S.C., Edirisinghe, R., Bongso, A., Ratnam, S. (1992). The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos. Fertility and Sterility 58 (6) : 1250-1253. ScholarBank@NUS Repository.
Abstract: Bicarbonate-buffered HTF medium, Medicult, and T6 are as effective as PB1 medium when used in combination with DMSO in ultrarapid freezing of two-cell mouse embryos. However, the use of phosphate-buffered T6 results in reduced in vitro development and inner cell mass size as compared with bicarbonate- and Hepes-buffered T6 when used with 3.5 M of DMSO. Hence, the use of this media for ultrarapid freezing should be avoided when this concentration of DMSO is used.
Source Title: Fertility and Sterility
URI: http://scholarbank.nus.edu.sg/handle/10635/133726
ISSN: 00150282
Appears in Collections:Staff Publications

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