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|Title:||The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos|
|Authors:||Vasuthevan, S. |
|Source:||Vasuthevan, S., Ng, S.C., Edirisinghe, R., Bongso, A., Ratnam, S. (1992). The evaluation of various culture media in combination with dimethylsulfoxide for ultrarapid freezing of murine embryos. Fertility and Sterility 58 (6) : 1250-1253. ScholarBank@NUS Repository.|
|Abstract:||Bicarbonate-buffered HTF medium, Medicult, and T6 are as effective as PB1 medium when used in combination with DMSO in ultrarapid freezing of two-cell mouse embryos. However, the use of phosphate-buffered T6 results in reduced in vitro development and inner cell mass size as compared with bicarbonate- and Hepes-buffered T6 when used with 3.5 M of DMSO. Hence, the use of this media for ultrarapid freezing should be avoided when this concentration of DMSO is used.|
|Source Title:||Fertility and Sterility|
|Appears in Collections:||Staff Publications|
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