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Title: Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation
Authors: Yao, M.
Goult, B.T.
Chen, H. 
Cong, P.
Sheetz, M.P. 
Yan, J. 
Issue Date: 9-Apr-2014
Citation: Yao, M., Goult, B.T., Chen, H., Cong, P., Sheetz, M.P., Yan, J. (2014-04-09). Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation. Scientific Reports 4 : -. ScholarBank@NUS Repository.
Abstract: The force-dependent interaction between talin and vinculin plays a crucial role in the initiation and growth of focal adhesions. Here we use magnetic tweezers to characterise the mechano-sensitive compact N-terminal region of the talin rod, and show that the three helical bundles R1-R3 in this region unfold in three distinct steps consistent with the domains unfolding independently. Mechanical stretching of talin R1-R3 enhances its binding to vinculin and vinculin binding inhibits talin refolding after force is released. Mutations that stabilize R3 identify it as the initial mechano-sensing domain in talin, unfolding at a ̂1/45a €...pN, suggesting that 5a €...pN is the force threshold for vinculin binding and adhesion progression.
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/srep04610
Appears in Collections:Staff Publications

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