Please use this identifier to cite or link to this item:
|Title:||Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation|
|Citation:||Yao, M., Goult, B.T., Chen, H., Cong, P., Sheetz, M.P., Yan, J. (2014-04-09). Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation. Scientific Reports 4 : -. ScholarBank@NUS Repository. https://doi.org/10.1038/srep04610|
|Abstract:||The force-dependent interaction between talin and vinculin plays a crucial role in the initiation and growth of focal adhesions. Here we use magnetic tweezers to characterise the mechano-sensitive compact N-terminal region of the talin rod, and show that the three helical bundles R1-R3 in this region unfold in three distinct steps consistent with the domains unfolding independently. Mechanical stretching of talin R1-R3 enhances its binding to vinculin and vinculin binding inhibits talin refolding after force is released. Mutations that stabilize R3 identify it as the initial mechano-sensing domain in talin, unfolding at a ̂1/45a €...pN, suggesting that 5a €...pN is the force threshold for vinculin binding and adhesion progression.|
|Source Title:||Scientific Reports|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 6, 2018
WEB OF SCIENCETM
checked on Nov 28, 2018
checked on Oct 19, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.