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|Title:||Real-time liquid crystal pH sensor for monitoring enzymatic activities of penicillinase||Authors:||Bi, X.
|Issue Date:||12-Sep-2009||Citation:||Bi, X., Hartono, D., Yang, K.-L. (2009-09-12). Real-time liquid crystal pH sensor for monitoring enzymatic activities of penicillinase. Advanced Functional Materials 19 (23) : 3760-3765. ScholarBank@NUS Repository.||Abstract:||A liquid crystal (LC)-based pH sensor for real-time monitoring of changes in localized pH values near a solid surface is reported, along with its application for the detection of enzymatic activities. It is found that 4-cyano-4'-pentylbiphenyl 5CB), when doped with 4'-pentyl-biphenyl-4carboxylic acid (PBA), shows a bright-to-dark optical response to a very small change in pH (from 6.9 to 7.0). The pH-driven optical response can be explained by using orientational transitions of 5CB induced by the protonation and deprotonation of PBA at the aqueous/LC interface. Because of its high pH sensitivity, the LC-based sensor is further exploited for monitoring local pH changes resulting from enzymatic reactions. As a proof of concept, the hydrolysis of penicillin C by surface-immobilized penicillinase is monitored using the system, even when the concentration of penicillin G is as low as 1 nM. This type of LC-based sensor may find potential utilities in high-throughput screening of enzyme substrates and enzyme inhibitors. © 2009 WILEY-VCH Verlag GmbH &. Co. KCaA.||Source Title:||Advanced Functional Materials||URI:||http://scholarbank.nus.edu.sg/handle/10635/89983||ISSN:||1616301X|
|Appears in Collections:||Staff Publications|
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