Please use this identifier to cite or link to this item:
https://doi.org/10.1002/adfm.200900823
Title: | Real-time liquid crystal pH sensor for monitoring enzymatic activities of penicillinase |
Authors: | Bi, X. Hartono, D. Yang, K.-L. |
Issue Date: | 12-Sep-2009 |
Source: | Bi, X., Hartono, D., Yang, K.-L. (2009-09-12). Real-time liquid crystal pH sensor for monitoring enzymatic activities of penicillinase. Advanced Functional Materials 19 (23) : 3760-3765. ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.200900823 |
Abstract: | A liquid crystal (LC)-based pH sensor for real-time monitoring of changes in localized pH values near a solid surface is reported, along with its application for the detection of enzymatic activities. It is found that 4-cyano-4'-pentylbiphenyl 5CB), when doped with 4'-pentyl-biphenyl-4carboxylic acid (PBA), shows a bright-to-dark optical response to a very small change in pH (from 6.9 to 7.0). The pH-driven optical response can be explained by using orientational transitions of 5CB induced by the protonation and deprotonation of PBA at the aqueous/LC interface. Because of its high pH sensitivity, the LC-based sensor is further exploited for monitoring local pH changes resulting from enzymatic reactions. As a proof of concept, the hydrolysis of penicillin C by surface-immobilized penicillinase is monitored using the system, even when the concentration of penicillin G is as low as 1 nM. This type of LC-based sensor may find potential utilities in high-throughput screening of enzyme substrates and enzyme inhibitors. © 2009 WILEY-VCH Verlag GmbH &. Co. KCaA. |
Source Title: | Advanced Functional Materials |
URI: | http://scholarbank.nus.edu.sg/handle/10635/89983 |
ISSN: | 1616301X |
DOI: | 10.1002/adfm.200900823 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
SCOPUSTM
Citations
79
checked on Mar 21, 2018
WEB OF SCIENCETM
Citations
77
checked on Mar 21, 2018
Page view(s)
33
checked on Apr 20, 2018
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.