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|Title:||Detecting DNA targets through the formation of DNA/CTAB complex and its interactions with liquid crystals||Authors:||Lai, S.L.
|Issue Date:||21-Aug-2011||Citation:||Lai, S.L., Yang, K.-L. (2011-08-21). Detecting DNA targets through the formation of DNA/CTAB complex and its interactions with liquid crystals. Analyst 136 (16) : 3329-3334. ScholarBank@NUS Repository. https://doi.org/10.1039/c1an15173h||Abstract:||In this paper, we report the formation of a DNA/cetyl trimethylammonium bromide (CTAB) complex on a solid surface and its interaction with a thin layer of liquid crystals (LC) supported on the surface. Our results show that when the surface is decorated with DNA only, the LC gives a bright image, but when the surface is decorated with the DNA/CTAB complex, the LC becomes dark when the surface density of CTAB is above 5.25 ± 0.13 × 10 13/cm 2. To exploit this phenomenon for detecting DNA targets, we used a surface decorated with electroneutral PNA probes for capturing DNA targets, and then treated the surface with 0.1 mM of CTAB. In the presence of DNA targets, a PNA/DNA/CTAB complex is formed and that leads to a dark image on the thin layer of the LC supported on the surface. Moreover, DNA targets with a complementary, 1-base mismatch and non-complementary sequence can be differentiated by using this method. This study provides a new principle for the label-free detection of DNA targets without any fluorescent labels. © 2011 The Royal Society of Chemistry.||Source Title:||Analyst||URI:||http://scholarbank.nus.edu.sg/handle/10635/88750||ISSN:||00032654||DOI:||10.1039/c1an15173h|
|Appears in Collections:||Staff Publications|
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