Please use this identifier to cite or link to this item: https://doi.org/10.1039/c1an15173h
Title: Detecting DNA targets through the formation of DNA/CTAB complex and its interactions with liquid crystals
Authors: Lai, S.L.
Yang, K.-L. 
Issue Date: 21-Aug-2011
Source: Lai, S.L., Yang, K.-L. (2011-08-21). Detecting DNA targets through the formation of DNA/CTAB complex and its interactions with liquid crystals. Analyst 136 (16) : 3329-3334. ScholarBank@NUS Repository. https://doi.org/10.1039/c1an15173h
Abstract: In this paper, we report the formation of a DNA/cetyl trimethylammonium bromide (CTAB) complex on a solid surface and its interaction with a thin layer of liquid crystals (LC) supported on the surface. Our results show that when the surface is decorated with DNA only, the LC gives a bright image, but when the surface is decorated with the DNA/CTAB complex, the LC becomes dark when the surface density of CTAB is above 5.25 ± 0.13 × 10 13/cm 2. To exploit this phenomenon for detecting DNA targets, we used a surface decorated with electroneutral PNA probes for capturing DNA targets, and then treated the surface with 0.1 mM of CTAB. In the presence of DNA targets, a PNA/DNA/CTAB complex is formed and that leads to a dark image on the thin layer of the LC supported on the surface. Moreover, DNA targets with a complementary, 1-base mismatch and non-complementary sequence can be differentiated by using this method. This study provides a new principle for the label-free detection of DNA targets without any fluorescent labels. © 2011 The Royal Society of Chemistry.
Source Title: Analyst
URI: http://scholarbank.nus.edu.sg/handle/10635/88750
ISSN: 00032654
DOI: 10.1039/c1an15173h
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on Feb 22, 2018

WEB OF SCIENCETM
Citations

12
checked on Jan 24, 2018

Page view(s)

21
checked on Feb 19, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.