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Title: Detecting DNA targets through the formation of DNA/CTAB complex and its interactions with liquid crystals
Authors: Lai, S.L.
Yang, K.-L. 
Issue Date: 21-Aug-2011
Citation: Lai, S.L., Yang, K.-L. (2011-08-21). Detecting DNA targets through the formation of DNA/CTAB complex and its interactions with liquid crystals. Analyst 136 (16) : 3329-3334. ScholarBank@NUS Repository.
Abstract: In this paper, we report the formation of a DNA/cetyl trimethylammonium bromide (CTAB) complex on a solid surface and its interaction with a thin layer of liquid crystals (LC) supported on the surface. Our results show that when the surface is decorated with DNA only, the LC gives a bright image, but when the surface is decorated with the DNA/CTAB complex, the LC becomes dark when the surface density of CTAB is above 5.25 ± 0.13 × 10 13/cm 2. To exploit this phenomenon for detecting DNA targets, we used a surface decorated with electroneutral PNA probes for capturing DNA targets, and then treated the surface with 0.1 mM of CTAB. In the presence of DNA targets, a PNA/DNA/CTAB complex is formed and that leads to a dark image on the thin layer of the LC supported on the surface. Moreover, DNA targets with a complementary, 1-base mismatch and non-complementary sequence can be differentiated by using this method. This study provides a new principle for the label-free detection of DNA targets without any fluorescent labels. © 2011 The Royal Society of Chemistry.
Source Title: Analyst
ISSN: 00032654
DOI: 10.1039/c1an15173h
Appears in Collections:Staff Publications

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