Please use this identifier to cite or link to this item:
|Title:||Intein-mediated biotinylation of proteins and its application in a protein microarray||Authors:||Lesaicherre, M.-L.
|Issue Date:||31-Jul-2002||Citation:||Lesaicherre, M.-L., Lue, R.Y.P., Chen, G.Y.J., Zhu, Q., Yao, S.Q. (2002-07-31). Intein-mediated biotinylation of proteins and its application in a protein microarray. Journal of the American Chemical Society 124 (30) : 8768-8769. ScholarBank@NUS Repository. https://doi.org/10.1021/ja0265963||Abstract:||We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays. Copyright © 2002 American Chemical Society.||Source Title:||Journal of the American Chemical Society||URI:||http://scholarbank.nus.edu.sg/handle/10635/76388||ISSN:||00027863||DOI:||10.1021/ja0265963|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Apr 10, 2021
WEB OF SCIENCETM
checked on Apr 2, 2021
checked on Apr 12, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.