Intein-mediated biotinylation of proteins and its application in a protein microarray

Please use this identifier to cite or link to this item: https://doi.org/10.1021/ja0265963

Title:	Intein-mediated biotinylation of proteins and its application in a protein microarray
Authors:	Lesaicherre, M.-L. Lue, R.Y.P. Chen, G.Y.J. Zhu, Q. Yao, S.Q.
Issue Date:	31-Jul-2002
Citation:	Lesaicherre, M.-L., Lue, R.Y.P., Chen, G.Y.J., Zhu, Q., Yao, S.Q. (2002-07-31). Intein-mediated biotinylation of proteins and its application in a protein microarray. Journal of the American Chemical Society 124 (30) : 8768-8769. ScholarBank@NUS Repository. https://doi.org/10.1021/ja0265963
Abstract:	We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays. Copyright © 2002 American Chemical Society.
Source Title:	Journal of the American Chemical Society
URI:	http://scholarbank.nus.edu.sg/handle/10635/76388
ISSN:	00027863
DOI:	10.1021/ja0265963
Appears in Collections:	Staff Publications

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