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|Title:||Intein-mediated biotinylation of proteins and its application in a protein microarray|
|Authors:||Lesaicherre, M.-L. |
|Citation:||Lesaicherre, M.-L., Lue, R.Y.P., Chen, G.Y.J., Zhu, Q., Yao, S.Q. (2002-07-31). Intein-mediated biotinylation of proteins and its application in a protein microarray. Journal of the American Chemical Society 124 (30) : 8768-8769. ScholarBank@NUS Repository. https://doi.org/10.1021/ja0265963|
|Abstract:||We report here the first example using an intein-mediated expression system to generate biotinylated proteins suitable for immobilization onto avidin-functionalized glass slides. With this novel array, proteins are site-specifically immobilized on the glass surface and are able to retain their native activity. The advantage of the avidin/biotin linkage over his-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety of chemical conditions, which makes this new protein array compatible with most biological assays. Copyright © 2002 American Chemical Society.|
|Source Title:||Journal of the American Chemical Society|
|Appears in Collections:||Staff Publications|
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