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https://doi.org/10.1016/j.bmcl.2004.09.083
Title: | Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo | Authors: | Tan, L.-P. Lue, R.Y.P. Chen, G.Y.J. Yao, S.Q. |
Keywords: | Intein Protein array Protein biotinylation Proteomics |
Issue Date: | 20-Dec-2004 | Citation: | Tan, L.-P., Lue, R.Y.P., Chen, G.Y.J., Yao, S.Q. (2004-12-20). Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorganic and Medicinal Chemistry Letters 14 (24) : 6067-6070. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.083 | Abstract: | One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. © 2004 Elsevier Ltd. All rights reserved. | Source Title: | Bioorganic and Medicinal Chemistry Letters | URI: | http://scholarbank.nus.edu.sg/handle/10635/76357 | ISSN: | 0960894X | DOI: | 10.1016/j.bmcl.2004.09.083 |
Appears in Collections: | Staff Publications |
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