Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2004.09.083
Title: Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo
Authors: Tan, L.-P. 
Lue, R.Y.P.
Chen, G.Y.J. 
Yao, S.Q. 
Keywords: Intein
Protein array
Protein biotinylation
Proteomics
Issue Date: 20-Dec-2004
Citation: Tan, L.-P., Lue, R.Y.P., Chen, G.Y.J., Yao, S.Q. (2004-12-20). Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorganic and Medicinal Chemistry Letters 14 (24) : 6067-6070. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.083
Abstract: One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. © 2004 Elsevier Ltd. All rights reserved.
Source Title: Bioorganic and Medicinal Chemistry Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/76357
ISSN: 0960894X
DOI: 10.1016/j.bmcl.2004.09.083
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