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|Title:||Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo|
|Authors:||Tan, L.-P. |
|Citation:||Tan, L.-P., Lue, R.Y.P., Chen, G.Y.J., Yao, S.Q. (2004-12-20). Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorganic and Medicinal Chemistry Letters 14 (24) : 6067-6070. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.083|
|Abstract:||One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. © 2004 Elsevier Ltd. All rights reserved.|
|Source Title:||Bioorganic and Medicinal Chemistry Letters|
|Appears in Collections:||Staff Publications|
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