Expanding the scope of site-specific protein biotinylation strategies using small molecules

Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2004.09.056

Title:	Expanding the scope of site-specific protein biotinylation strategies using small molecules
Authors:	Tan, L.-P. Chen, G.Y.J. Yao, S.Q.
Keywords:	Biotinylation Cell-free protein expression Microarray Protein Puromycin
Issue Date:	6-Dec-2004
Citation:	Tan, L.-P., Chen, G.Y.J., Yao, S.Q. (2004-12-06). Expanding the scope of site-specific protein biotinylation strategies using small molecules. Bioorganic and Medicinal Chemistry Letters 14 (23) : 5735-5738. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.056
Abstract:	We present a new approach to site-specifically biotinylate protein in a cell-free protein synthesis system with puromycin-containing small molecules. With this new method, biotinylated proteins were generated from the DNA templates in a matter of hours, making it useful for protein microarray generation. We also validated that the method is compatible with other high-throughput cloning/proteomics methods. © 2004 Elsevier Ltd. All rights reserved.
Source Title:	Bioorganic and Medicinal Chemistry Letters
URI:	http://scholarbank.nus.edu.sg/handle/10635/76151
ISSN:	0960894X
DOI:	10.1016/j.bmcl.2004.09.056
Appears in Collections:	Staff Publications

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