Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bmcl.2004.09.056
Title: Expanding the scope of site-specific protein biotinylation strategies using small molecules
Authors: Tan, L.-P. 
Chen, G.Y.J. 
Yao, S.Q. 
Keywords: Biotinylation
Cell-free protein expression
Microarray
Protein
Puromycin
Issue Date: 6-Dec-2004
Citation: Tan, L.-P., Chen, G.Y.J., Yao, S.Q. (2004-12-06). Expanding the scope of site-specific protein biotinylation strategies using small molecules. Bioorganic and Medicinal Chemistry Letters 14 (23) : 5735-5738. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.056
Abstract: We present a new approach to site-specifically biotinylate protein in a cell-free protein synthesis system with puromycin-containing small molecules. With this new method, biotinylated proteins were generated from the DNA templates in a matter of hours, making it useful for protein microarray generation. We also validated that the method is compatible with other high-throughput cloning/proteomics methods. © 2004 Elsevier Ltd. All rights reserved.
Source Title: Bioorganic and Medicinal Chemistry Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/76151
ISSN: 0960894X
DOI: 10.1016/j.bmcl.2004.09.056
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