Activity-based high-throughput screening of enzymes by using a DNA microarray

Abstract

The expression display method is developed, which combines two techniques, ribosome display and DNA microarray, to express a mixture of proteins from their corresponding cDNA. Upon functional selection using an activity-based probe, multiple target enzymes are simultaneously isolated and subsequently identified by hybridization to a DNA microarray (see scheme; RT = reverse transcription, PCR = polymerase chain reaction). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.