Please use this identifier to cite or link to this item: https://doi.org/10.1002/anie.200461612
Title: Activity-based high-throughput screening of enzymes by using a DNA microarray
Authors: Hu, Y.
Chen, G.Y.J. 
Yao, S.Q. 
Keywords: Analytical methods
DNA microarrays
Enzymes
Proteomics
Ribosome display
Issue Date: 4-Feb-2005
Citation: Hu, Y., Chen, G.Y.J., Yao, S.Q. (2005-02-04). Activity-based high-throughput screening of enzymes by using a DNA microarray. Angewandte Chemie - International Edition 44 (7) : 1048-1053. ScholarBank@NUS Repository. https://doi.org/10.1002/anie.200461612
Abstract: The expression display method is developed, which combines two techniques, ribosome display and DNA microarray, to express a mixture of proteins from their corresponding cDNA. Upon functional selection using an activity-based probe, multiple target enzymes are simultaneously isolated and subsequently identified by hybridization to a DNA microarray (see scheme; RT = reverse transcription, PCR = polymerase chain reaction). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Angewandte Chemie - International Edition
URI: http://scholarbank.nus.edu.sg/handle/10635/75523
ISSN: 14337851
DOI: 10.1002/anie.200461612
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