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Title: Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note
Authors: Zeugolis, D.I. 
Li, B. 
Lareu, R.R.
Chan, C.K. 
Raghunath, M. 
Keywords: Collagen nano-fibres
Collagen solubility
Issue Date: 1-Oct-2008
Citation: Zeugolis, D.I., Li, B., Lareu, R.R., Chan, C.K., Raghunath, M. (2008-10-01). Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note. Journal of Biomaterials Science, Polymer Edition 19 (10) : 1307-1317. ScholarBank@NUS Repository.
Abstract: Collagen is the main component of the extra-cellular matrix and has been utilised for numerous clinical applications in many forms and products. However, since collagen remains a natural animal-derived biopolymer, variation between batches should be addressed and minimised to ensure reproducibility of the fabrication process. Recently, electro-spinning of collagen has been introduced as a leading technique for the production of bio-mimetic nano-scale scaffolds for tissue-engineering applications. However, no protocols are available that would allow comparisons of the quality of different collagen raw materials prior to the electro-spinning process. In order to bridge this gap we assessed the solubility of various freeze-dried collagens in 0.5 M acetic acid and analysed the solved collagen by gel electrophoresis. We show that raw material of limited solubility in acetic acid will not render high quality electro-spun nano-fibres using hexafluoropropanol. In particular, insoluble collagen directly failed to produce nano-fibres, collagen of reduced solubility produced fused nano-fibres with limited inter-nano-fibre space, whilst purified type-I collagen of high solubility produced smooth, reproducible nano-fibres. Gel electrophoresis confirmed the amount of solubility, as well as qualitative differences in terms of collagen cross-links and collagen types. We recommend this simple and fast step to save costs and to enhance control over the electro-spinning process of collagen. Furthermore, we believe that the solubility test should be introduced prior to any collagenous matrix preparation in order to ensure reproducibility and accuracy. © 2008 VSP.
Source Title: Journal of Biomaterials Science, Polymer Edition
ISSN: 09205063
DOI: 10.1163/156856208786052344
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