Please use this identifier to cite or link to this item: https://doi.org/10.1163/156856208786052344
Title: Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note
Authors: Zeugolis, D.I. 
Li, B. 
Lareu, R.R.
Chan, C.K. 
Raghunath, M. 
Keywords: Collagen nano-fibres
Collagen solubility
Electro-spinning
SDS-PAGE
Issue Date: 1-Oct-2008
Source: Zeugolis, D.I., Li, B., Lareu, R.R., Chan, C.K., Raghunath, M. (2008-10-01). Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note. Journal of Biomaterials Science, Polymer Edition 19 (10) : 1307-1317. ScholarBank@NUS Repository. https://doi.org/10.1163/156856208786052344
Abstract: Collagen is the main component of the extra-cellular matrix and has been utilised for numerous clinical applications in many forms and products. However, since collagen remains a natural animal-derived biopolymer, variation between batches should be addressed and minimised to ensure reproducibility of the fabrication process. Recently, electro-spinning of collagen has been introduced as a leading technique for the production of bio-mimetic nano-scale scaffolds for tissue-engineering applications. However, no protocols are available that would allow comparisons of the quality of different collagen raw materials prior to the electro-spinning process. In order to bridge this gap we assessed the solubility of various freeze-dried collagens in 0.5 M acetic acid and analysed the solved collagen by gel electrophoresis. We show that raw material of limited solubility in acetic acid will not render high quality electro-spun nano-fibres using hexafluoropropanol. In particular, insoluble collagen directly failed to produce nano-fibres, collagen of reduced solubility produced fused nano-fibres with limited inter-nano-fibre space, whilst purified type-I collagen of high solubility produced smooth, reproducible nano-fibres. Gel electrophoresis confirmed the amount of solubility, as well as qualitative differences in terms of collagen cross-links and collagen types. We recommend this simple and fast step to save costs and to enhance control over the electro-spinning process of collagen. Furthermore, we believe that the solubility test should be introduced prior to any collagenous matrix preparation in order to ensure reproducibility and accuracy. © 2008 VSP.
Source Title: Journal of Biomaterials Science, Polymer Edition
URI: http://scholarbank.nus.edu.sg/handle/10635/66969
ISSN: 09205063
DOI: 10.1163/156856208786052344
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

16
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

12
checked on Nov 13, 2017

Page view(s)

44
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.