Please use this identifier to cite or link to this item: https://doi.org/10.1163/156856208786052344
DC FieldValue
dc.titleCollagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note
dc.contributor.authorZeugolis, D.I.
dc.contributor.authorLi, B.
dc.contributor.authorLareu, R.R.
dc.contributor.authorChan, C.K.
dc.contributor.authorRaghunath, M.
dc.date.accessioned2014-06-17T09:42:51Z
dc.date.available2014-06-17T09:42:51Z
dc.date.issued2008-10-01
dc.identifier.citationZeugolis, D.I., Li, B., Lareu, R.R., Chan, C.K., Raghunath, M. (2008-10-01). Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication. A technical note. Journal of Biomaterials Science, Polymer Edition 19 (10) : 1307-1317. ScholarBank@NUS Repository. https://doi.org/10.1163/156856208786052344
dc.identifier.issn09205063
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/66969
dc.description.abstractCollagen is the main component of the extra-cellular matrix and has been utilised for numerous clinical applications in many forms and products. However, since collagen remains a natural animal-derived biopolymer, variation between batches should be addressed and minimised to ensure reproducibility of the fabrication process. Recently, electro-spinning of collagen has been introduced as a leading technique for the production of bio-mimetic nano-scale scaffolds for tissue-engineering applications. However, no protocols are available that would allow comparisons of the quality of different collagen raw materials prior to the electro-spinning process. In order to bridge this gap we assessed the solubility of various freeze-dried collagens in 0.5 M acetic acid and analysed the solved collagen by gel electrophoresis. We show that raw material of limited solubility in acetic acid will not render high quality electro-spun nano-fibres using hexafluoropropanol. In particular, insoluble collagen directly failed to produce nano-fibres, collagen of reduced solubility produced fused nano-fibres with limited inter-nano-fibre space, whilst purified type-I collagen of high solubility produced smooth, reproducible nano-fibres. Gel electrophoresis confirmed the amount of solubility, as well as qualitative differences in terms of collagen cross-links and collagen types. We recommend this simple and fast step to save costs and to enhance control over the electro-spinning process of collagen. Furthermore, we believe that the solubility test should be introduced prior to any collagenous matrix preparation in order to ensure reproducibility and accuracy. © 2008 VSP.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1163/156856208786052344
dc.sourceScopus
dc.subjectCollagen nano-fibres
dc.subjectCollagen solubility
dc.subjectElectro-spinning
dc.subjectSDS-PAGE
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1163/156856208786052344
dc.description.sourcetitleJournal of Biomaterials Science, Polymer Edition
dc.description.volume19
dc.description.issue10
dc.description.page1307-1317
dc.description.codenJBSEE
dc.identifier.isiut000262267500004
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

28
checked on Dec 11, 2019

WEB OF SCIENCETM
Citations

22
checked on Dec 3, 2019

Page view(s)

87
checked on Dec 1, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.