Please use this identifier to cite or link to this item:
|Title:||Chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen, Der p 1 for oral vaccination in mice||Authors:||Chew, J.L.
Der p 1
|Issue Date:||2003||Citation:||Chew, J.L., Wolfowicz, C.B., Chua, K.Y., Mao, H.-Q., Leong, K.W. (2003). Chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen, Der p 1 for oral vaccination in mice. Vaccine 21 (21-22) : 2720-2729. ScholarBank@NUS Repository. https://doi.org/10.1016/S0264-410X(03)00228-7||Abstract:||Our previous studies indicated that intramuscular (i.m.) immunisation with full length Der p 1 cDNA induced significant humoral response to the left domain (approximately corresponding to amino acids 1-116) but not to the right domain (approximately corresponding to amino acids 117-222) of Der p 1 allergen. This study explored the use of chitosan-DNA nanoparticles for oral immunisation to induce immune responses specific to both the left and right domains of Der p 1. DNA constructs pDer p 1 (1-222) and pDer p 1 (114-222) were complexed with chitosan and delivered orally followed by an i.m. injection of pDer p 1 (1-222) 13 weeks later. Such approach has successfully primed Th1-skewed immune responses against both domains of Der p 1. This strategy could be further optimised for more efficacious gene vaccination for full length Der p 1. © 2003 Elsevier Science Ltd. All rights reserved.||Source Title:||Vaccine||URI:||http://scholarbank.nus.edu.sg/handle/10635/48156||ISSN:||0264410X||DOI:||10.1016/S0264-410X(03)00228-7|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 4, 2019
WEB OF SCIENCETM
checked on Nov 27, 2019
checked on Dec 2, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.