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Title: Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts
Authors: Swamy, S.M.K.
Lu, J.
Achuth, H.N.
Moochhala, S.
Tan, P.
Zhu, Y.Z. 
Keywords: Cell proliferation
Gene expression
Wound healing
Issue Date: 2004
Citation: Swamy, S.M.K., Lu, J., Achuth, H.N., Moochhala, S., Tan, P., Zhu, Y.Z. (2004). Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts. Biochemical and Biophysical Research Communications 314 (3) : 661-666. ScholarBank@NUS Repository.
Abstract: Wound healing is a complex process involving a number of related genes and receptors. Using cDNA microarrays, we explored the global gene expression profile of phenytoin (20μg/ml) induced changes to human dermal fibroblasts. Microarray data analysis revealed ∼1500 genes were differentially expressed by 2.5-fold. At 3, 6, 12, and 24h, the transcripts of the major growth factors involved in wound healing and their receptors were increased. This was further confirmed by RT-PCR. Genes encoding other proteins with roles in signal transduction (NFκB), extracellular matrix (MMP1) including type I collagen, fibronectin, and laminin were strongly induced at 6h and onwards. Genes involved in cell cycle regulation (CCND1 and CDKN1A) were down-regulated consistent with our finding that phenytoin per se did not have cell proliferation activity. Notably, phenytoin accelerates the autocrine and paracrine activity of growth factors by up-regulating the related receptors. © 2003 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2003.12.146
Appears in Collections:Staff Publications

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