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|Title:||Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts||Authors:||Swamy, S.M.K.
|Issue Date:||2004||Citation:||Swamy, S.M.K., Lu, J., Achuth, H.N., Moochhala, S., Tan, P., Zhu, Y.Z. (2004). Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts. Biochemical and Biophysical Research Communications 314 (3) : 661-666. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2003.12.146||Abstract:||Wound healing is a complex process involving a number of related genes and receptors. Using cDNA microarrays, we explored the global gene expression profile of phenytoin (20μg/ml) induced changes to human dermal fibroblasts. Microarray data analysis revealed ∼1500 genes were differentially expressed by 2.5-fold. At 3, 6, 12, and 24h, the transcripts of the major growth factors involved in wound healing and their receptors were increased. This was further confirmed by RT-PCR. Genes encoding other proteins with roles in signal transduction (NFκB), extracellular matrix (MMP1) including type I collagen, fibronectin, and laminin were strongly induced at 6h and onwards. Genes involved in cell cycle regulation (CCND1 and CDKN1A) were down-regulated consistent with our finding that phenytoin per se did not have cell proliferation activity. Notably, phenytoin accelerates the autocrine and paracrine activity of growth factors by up-regulating the related receptors. © 2003 Elsevier Inc. All rights reserved.||Source Title:||Biochemical and Biophysical Research Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/32199||ISSN:||0006291X||DOI:||10.1016/j.bbrc.2003.12.146|
|Appears in Collections:||Staff Publications|
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