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Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2003.12.146
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dc.titleRole of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts
dc.contributor.authorSwamy, S.M.K.
dc.contributor.authorLu, J.
dc.contributor.authorAchuth, H.N.
dc.contributor.authorMoochhala, S.
dc.contributor.authorTan, P.
dc.contributor.authorZhu, Y.Z.
dc.date.accessioned2012-04-02T07:43:56Z
dc.date.available2012-04-02T07:43:56Z
dc.date.issued2004
dc.identifier.citationSwamy, S.M.K., Lu, J., Achuth, H.N., Moochhala, S., Tan, P., Zhu, Y.Z. (2004). Role of phenytoin in wound healing: Microarray analysis of early transcriptional responses in human dermal fibroblasts. Biochemical and Biophysical Research Communications 314 (3) : 661-666. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2003.12.146
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/32199
dc.description.abstractWound healing is a complex process involving a number of related genes and receptors. Using cDNA microarrays, we explored the global gene expression profile of phenytoin (20μg/ml) induced changes to human dermal fibroblasts. Microarray data analysis revealed ∼1500 genes were differentially expressed by 2.5-fold. At 3, 6, 12, and 24h, the transcripts of the major growth factors involved in wound healing and their receptors were increased. This was further confirmed by RT-PCR. Genes encoding other proteins with roles in signal transduction (NFκB), extracellular matrix (MMP1) including type I collagen, fibronectin, and laminin were strongly induced at 6h and onwards. Genes involved in cell cycle regulation (CCND1 and CDKN1A) were down-regulated consistent with our finding that phenytoin per se did not have cell proliferation activity. Notably, phenytoin accelerates the autocrine and paracrine activity of growth factors by up-regulating the related receptors. © 2003 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2003.12.146
dc.sourceScopus
dc.subjectCell proliferation
dc.subjectFibroblast
dc.subjectGene expression
dc.subjectGingival
dc.subjectHyperplasia
dc.subjectMicroarrays
dc.subjectPhenytoin
dc.subjectSkin
dc.subjectWound healing
dc.subjectWounds
dc.typeArticle
dc.contributor.departmentPHARMACOLOGY
dc.description.doi10.1016/j.bbrc.2003.12.146
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume314
dc.description.issue3
dc.description.page661-666
dc.description.codenBBRCA
dc.identifier.isiut000188613100001
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