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Title: Transformation of Endomyces fibuliger based on its gene for orotidine- 5'-phosphate decarboxylase
Authors: Ngan, W.Y.
Nga, B.H. 
Tan, H.M. 
Pridmore, D.
Mollet, B.
Keywords: Complementation
Integrative transformation
Rice wine
Issue Date: 2000
Citation: Ngan, W.Y., Nga, B.H., Tan, H.M., Pridmore, D., Mollet, B. (2000). Transformation of Endomyces fibuliger based on its gene for orotidine- 5'-phosphate decarboxylase. Gene 254 (1-2) : 97-103. ScholarBank@NUS Repository.
Abstract: Endomyces fibuliger is a yeast used in the production of Chinese rice wine. It secretes enzymes such as glucoamylase, α-amylase and acid protease. Very little is known of the genetics of E. fibuliger. In order to develop a transformation system for this yeast, orotidine-5'-phosphate decarboxylase mutant strains were obtained and characterized. Transformation of the E. fibuliger ura3 mutant F1 with an integrative plasmid that carried the wild- type URA3 gene of E. fibuliger gave complementation of this mutation. The E. fibuliger gene encodes the orotidine-5'-phosphate decarboxylase enzyme consisting of 266 amino acid residues with a 69.4% sequence identity with orotidine-5'-phosphate decarboxylase of Saccharomyces cerevisiae. Our finding that E. fibuliger URA3 complements the ura3 mutation in S. cerevisiae confirms that the URA3 gene of E. fibuliger encodes a protein that exerts a similar function. (C) 2000 Elsevier Science B.V.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/S0378-1119(00)00286-9
Appears in Collections:Staff Publications

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