Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1119(00)00286-9
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dc.titleTransformation of Endomyces fibuliger based on its gene for orotidine- 5'-phosphate decarboxylase
dc.contributor.authorNgan, W.Y.
dc.contributor.authorNga, B.H.
dc.contributor.authorTan, H.M.
dc.contributor.authorPridmore, D.
dc.contributor.authorMollet, B.
dc.date.accessioned2012-03-28T05:50:10Z
dc.date.available2012-03-28T05:50:10Z
dc.date.issued2000
dc.identifier.citationNgan, W.Y., Nga, B.H., Tan, H.M., Pridmore, D., Mollet, B. (2000). Transformation of Endomyces fibuliger based on its gene for orotidine- 5'-phosphate decarboxylase. Gene 254 (1-2) : 97-103. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(00)00286-9
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/31289
dc.description.abstractEndomyces fibuliger is a yeast used in the production of Chinese rice wine. It secretes enzymes such as glucoamylase, α-amylase and acid protease. Very little is known of the genetics of E. fibuliger. In order to develop a transformation system for this yeast, orotidine-5'-phosphate decarboxylase mutant strains were obtained and characterized. Transformation of the E. fibuliger ura3 mutant F1 with an integrative plasmid that carried the wild- type URA3 gene of E. fibuliger gave complementation of this mutation. The E. fibuliger gene encodes the orotidine-5'-phosphate decarboxylase enzyme consisting of 266 amino acid residues with a 69.4% sequence identity with orotidine-5'-phosphate decarboxylase of Saccharomyces cerevisiae. Our finding that E. fibuliger URA3 complements the ura3 mutation in S. cerevisiae confirms that the URA3 gene of E. fibuliger encodes a protein that exerts a similar function. (C) 2000 Elsevier Science B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1119(00)00286-9
dc.sourceScopus
dc.subjectComplementation
dc.subjectIntegrative transformation
dc.subjectRice wine
dc.subjectURA3
dc.subjectYeast
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/S0378-1119(00)00286-9
dc.description.sourcetitleGene
dc.description.volume254
dc.description.issue1-2
dc.description.page97-103
dc.description.codenGENED
dc.identifier.isiut000089210100011
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