Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1119(00)00286-9
Title: Transformation of Endomyces fibuliger based on its gene for orotidine- 5'-phosphate decarboxylase
Authors: Ngan, W.Y.
Nga, B.H. 
Tan, H.M. 
Pridmore, D.
Mollet, B.
Keywords: Complementation
Integrative transformation
Rice wine
URA3
Yeast
Issue Date: 2000
Source: Ngan, W.Y., Nga, B.H., Tan, H.M., Pridmore, D., Mollet, B. (2000). Transformation of Endomyces fibuliger based on its gene for orotidine- 5'-phosphate decarboxylase. Gene 254 (1-2) : 97-103. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(00)00286-9
Abstract: Endomyces fibuliger is a yeast used in the production of Chinese rice wine. It secretes enzymes such as glucoamylase, α-amylase and acid protease. Very little is known of the genetics of E. fibuliger. In order to develop a transformation system for this yeast, orotidine-5'-phosphate decarboxylase mutant strains were obtained and characterized. Transformation of the E. fibuliger ura3 mutant F1 with an integrative plasmid that carried the wild- type URA3 gene of E. fibuliger gave complementation of this mutation. The E. fibuliger gene encodes the orotidine-5'-phosphate decarboxylase enzyme consisting of 266 amino acid residues with a 69.4% sequence identity with orotidine-5'-phosphate decarboxylase of Saccharomyces cerevisiae. Our finding that E. fibuliger URA3 complements the ura3 mutation in S. cerevisiae confirms that the URA3 gene of E. fibuliger encodes a protein that exerts a similar function. (C) 2000 Elsevier Science B.V.
Source Title: Gene
URI: http://scholarbank.nus.edu.sg/handle/10635/31289
ISSN: 03781119
DOI: 10.1016/S0378-1119(00)00286-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

5
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

5
checked on Nov 23, 2017

Page view(s)

159
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.