Please use this identifier to cite or link to this item:
|Title:||Rab22B's role in trans-Golgi network membrane dynamics||Authors:||Ng, E.L.
trans-Golgi network (TGN)
|Issue Date:||2007||Citation:||Ng, E.L., Wang, Y., Tang, B.L. (2007). Rab22B's role in trans-Golgi network membrane dynamics. Biochemical and Biophysical Research Communications 361 (3) : 751-757. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2007.07.076||Abstract:||The small GTPase Rab22B (or Rab31) has been suspected to be involved in trafficking at trans-Golgi network. However, its exact cellular localization, tissue expression profile, and functions have not been uncharacterized. Specific antibody raised against Rab22B's protein revealed that Rab22B is brain-enriched, but is also present in substantial levels in spleen and intestine. In HeLa cells, endogenous Rab22B is largely associated with the trans-Golgi network (TGN). Over-expression of a GDP-binding mutant (Rab22BSN), but not wild-type Rab22B, specifically disrupts the TGN localization of TGN46, a dynamic marker which cycles between the TGN and the plasma membrane. The TGN resident membrane protein syntaxin 16, cis-Golgi markers such as GM130 and syntaxin 5, as well as the TGN/late endosome marker mannose 6-phosphate receptor (M6PR) are not affected by Rab22BSN, neither was endosomal-TGN transport of the Shiga toxin B subunit. The disruption of TGN46 staining by Rab22BSN could be specifically attributed to a domain at the C-terminal portion of Rab22B, where its sequence deviates the most from Rab22A. Over-expression of Rab22BSN inhibits the cell surface transport of the vesicular stomatitis virus G protein. Thus, Rab22B may have a role in anterograde exit from the TGN. © 2007 Elsevier Inc. All rights reserved.||Source Title:||Biochemical and Biophysical Research Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/28645||ISSN:||0006291X
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 14, 2020
WEB OF SCIENCETM
checked on Feb 6, 2020
checked on Feb 5, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.