Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2007.07.076
DC FieldValue
dc.titleRab22B's role in trans-Golgi network membrane dynamics
dc.contributor.authorNg, E.L.
dc.contributor.authorWang, Y.
dc.contributor.authorTang, B.L.
dc.date.accessioned2011-11-29T05:57:44Z
dc.date.available2011-11-29T05:57:44Z
dc.date.issued2007
dc.identifier.citationNg, E.L., Wang, Y., Tang, B.L. (2007). Rab22B's role in trans-Golgi network membrane dynamics. Biochemical and Biophysical Research Communications 361 (3) : 751-757. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2007.07.076
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28645
dc.description.abstractThe small GTPase Rab22B (or Rab31) has been suspected to be involved in trafficking at trans-Golgi network. However, its exact cellular localization, tissue expression profile, and functions have not been uncharacterized. Specific antibody raised against Rab22B's protein revealed that Rab22B is brain-enriched, but is also present in substantial levels in spleen and intestine. In HeLa cells, endogenous Rab22B is largely associated with the trans-Golgi network (TGN). Over-expression of a GDP-binding mutant (Rab22BSN), but not wild-type Rab22B, specifically disrupts the TGN localization of TGN46, a dynamic marker which cycles between the TGN and the plasma membrane. The TGN resident membrane protein syntaxin 16, cis-Golgi markers such as GM130 and syntaxin 5, as well as the TGN/late endosome marker mannose 6-phosphate receptor (M6PR) are not affected by Rab22BSN, neither was endosomal-TGN transport of the Shiga toxin B subunit. The disruption of TGN46 staining by Rab22BSN could be specifically attributed to a domain at the C-terminal portion of Rab22B, where its sequence deviates the most from Rab22A. Over-expression of Rab22BSN inhibits the cell surface transport of the vesicular stomatitis virus G protein. Thus, Rab22B may have a role in anterograde exit from the TGN. © 2007 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2007.07.076
dc.sourceScopus
dc.subjectRab22B
dc.subjectSmall GTPase
dc.subjecttrans-Golgi network (TGN)
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/j.bbrc.2007.07.076
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume361
dc.description.issue3
dc.description.page751-757
dc.description.codenBBRCA
dc.identifier.isiut000248993100031
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