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|Title:||Simple and sensitive liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of five Epimedium prenylflavonoids in rat sera||Authors:||Shen, P.
Liquid chromatography-mass spectrometry (LC-MS)
|Issue Date:||2009||Citation:||Shen, P., Wong, S.P., Li, J., Yong, E.L. (2009). Simple and sensitive liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of five Epimedium prenylflavonoids in rat sera. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877 (1-2) : 71-78. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2008.11.030||Abstract:||A rapid and sensitive method to separate and quantify icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera was developed using liquid chromatography-tandem mass spectrometry. Serum samples were extracted with ethyl acetate without further derivatization. Using coumestrol as an internal standard, calibration curves with good linearity (r2 > 0.99) within the concentration range of 0.78-12.5 nM for icariin, icaritin and desmethylicaritin, and 0.78-100 nM for icariside I and II, were obtained in the multiple reaction monitoring mode. For all analytes, the limits of detection and quantification were <1 nM and 1-2 nM, respectively. Inter- and intra-assay variabilities were <15% and accuracies were between 94% and 114%, respectively. This method was successfully applied to quantify levels of icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera after oral administration of an Epimedium preparation. © 2008 Elsevier B.V. All rights reserved.||Source Title:||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences||URI:||http://scholarbank.nus.edu.sg/handle/10635/26007||ISSN:||15700232||DOI:||10.1016/j.jchromb.2008.11.030|
|Appears in Collections:||Staff Publications|
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