Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2008.11.030
Title: Simple and sensitive liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of five Epimedium prenylflavonoids in rat sera
Authors: Shen, P. 
Wong, S.P.
Li, J. 
Yong, E.L. 
Keywords: Epimedium
Liquid chromatography-mass spectrometry (LC-MS)
Prenylflavonoids
Rat serum
Issue Date: 2009
Source: Shen, P., Wong, S.P., Li, J., Yong, E.L. (2009). Simple and sensitive liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of five Epimedium prenylflavonoids in rat sera. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877 (1-2) : 71-78. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2008.11.030
Abstract: A rapid and sensitive method to separate and quantify icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera was developed using liquid chromatography-tandem mass spectrometry. Serum samples were extracted with ethyl acetate without further derivatization. Using coumestrol as an internal standard, calibration curves with good linearity (r2 > 0.99) within the concentration range of 0.78-12.5 nM for icariin, icaritin and desmethylicaritin, and 0.78-100 nM for icariside I and II, were obtained in the multiple reaction monitoring mode. For all analytes, the limits of detection and quantification were <1 nM and 1-2 nM, respectively. Inter- and intra-assay variabilities were <15% and accuracies were between 94% and 114%, respectively. This method was successfully applied to quantify levels of icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera after oral administration of an Epimedium preparation. © 2008 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/26007
ISSN: 15700232
DOI: 10.1016/j.jchromb.2008.11.030
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

26
checked on Dec 18, 2017

WEB OF SCIENCETM
Citations

24
checked on Nov 22, 2017

Page view(s)

228
checked on Dec 17, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.