Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2008.11.030
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dc.titleSimple and sensitive liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of five Epimedium prenylflavonoids in rat sera
dc.contributor.authorShen, P.
dc.contributor.authorWong, S.P.
dc.contributor.authorLi, J.
dc.contributor.authorYong, E.L.
dc.date.accessioned2011-09-15T08:21:58Z
dc.date.available2011-09-15T08:21:58Z
dc.date.issued2009
dc.identifier.citationShen, P., Wong, S.P., Li, J., Yong, E.L. (2009). Simple and sensitive liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of five Epimedium prenylflavonoids in rat sera. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877 (1-2) : 71-78. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2008.11.030
dc.identifier.issn15700232
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/26007
dc.description.abstractA rapid and sensitive method to separate and quantify icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera was developed using liquid chromatography-tandem mass spectrometry. Serum samples were extracted with ethyl acetate without further derivatization. Using coumestrol as an internal standard, calibration curves with good linearity (r2 > 0.99) within the concentration range of 0.78-12.5 nM for icariin, icaritin and desmethylicaritin, and 0.78-100 nM for icariside I and II, were obtained in the multiple reaction monitoring mode. For all analytes, the limits of detection and quantification were <1 nM and 1-2 nM, respectively. Inter- and intra-assay variabilities were <15% and accuracies were between 94% and 114%, respectively. This method was successfully applied to quantify levels of icariin, icariside I, icariside II, icaritin and desmethylicaritin in rat sera after oral administration of an Epimedium preparation. © 2008 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jchromb.2008.11.030
dc.sourceScopus
dc.subjectEpimedium
dc.subjectLiquid chromatography-mass spectrometry (LC-MS)
dc.subjectPrenylflavonoids
dc.subjectRat serum
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.1016/j.jchromb.2008.11.030
dc.description.sourcetitleJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.description.volume877
dc.description.issue1-2
dc.description.page71-78
dc.identifier.isiut000262618200012
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