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Title: Engineering microenvironment for expansion of sensitive anchorage-dependent mammalian cells
Authors: Chia, S.-M.
Lin, P.-C.
Goh, C.-H.
Ng, M.-L. 
Yu, H. 
Quek, C.-H.
Xu, X.
Yin, C.
Mao, H.-Q. 
Leong, K.W.
Keywords: Anchorage-dependent mammalian cells
Cell expansion
Non-disruptive harvesting
Stem cell research
Tissue engineering
Issue Date: 2005
Citation: Chia, S.-M., Lin, P.-C., Goh, C.-H., Ng, M.-L., Yu, H., Quek, C.-H., Xu, X., Yin, C., Mao, H.-Q., Leong, K.W. (2005). Engineering microenvironment for expansion of sensitive anchorage-dependent mammalian cells. Journal of Biotechnology 118 (4) : 434-447. ScholarBank@NUS Repository.
Abstract: Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional culture and harvest system circumventing the conventional detachment requirements. Cells mixed with dilute cationic collagen were microencapsulated within an ultra-thin shell of synthetic polymers. The cationic collagen could rapidly form a conformal layer of collagen fibers around cells to support cell proliferation and functions. The collagen could be readily removed from cells with a buffer rinse after harvesting from the fragile microcapsules. The cells harvested from this system demonstrate improved attachment, morphology and functions over conventionally cultured cells, upon binding to ligand-conjugated polymer surfaces. The harvested cells can be re-encapsulated and allowed to proliferate again, or used immediately in applications. © 2005 Elsevier B.V. All rights reserved.
Source Title: Journal of Biotechnology
ISSN: 01681656
DOI: 10.1016/j.jbiotec.2005.05.012
Appears in Collections:Staff Publications

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