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https://doi.org/10.1016/j.xpro.2023.102338
| Title: | Protocol for identification and validation of IGF2BP1 target genes in pluripotent human embryonic carcinoma cells | Authors: | Myint, K Chuang, LSH Matsuo, J Ito, Y |
Keywords: | Cancer Cell Biology Cell-based Assays Molecular Biology |
Issue Date: | 16-Jun-2023 | Publisher: | Elsevier BV | Citation: | Myint, K, Chuang, LSH, Matsuo, J, Ito, Y (2023-06-16). Protocol for identification and validation of IGF2BP1 target genes in pluripotent human embryonic carcinoma cells. STAR Protocols 4 (2) : 102338-. ScholarBank@NUS Repository. https://doi.org/10.1016/j.xpro.2023.102338 | Abstract: | We present a detailed protocol to identify and validate IGF2BP1 target genes in pluripotent human embryonic carcinoma cells (NTERA-2). We first identify the target genes through RNA-immunoprecipitation (RIP) sequencing. We then validate the identified targets through the use of RIP-qPCR assays, determine the m6A status of target genes by m6A-IP, and perform functional validation by quantifying changes in mRNA or protein expression levels upon knockdown of IGF2BP1 or methyltransferases in NTERA-2. For complete details on the use and execution of this protocol, please refer to Myint et al. (2022).1 | Source Title: | STAR Protocols | URI: | https://scholarbank.nus.edu.sg/handle/10635/248782 | ISSN: | 2666-1667 | DOI: | 10.1016/j.xpro.2023.102338 |
| Appears in Collections: | Staff Publications Elements Staff Publications |
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| File | Description | Size | Format | Access Settings | Version | |
|---|---|---|---|---|---|---|
| Protocol for identification and validation of IGF2BP1 target genes in pluripotent human embryonic carcinoma cells.pdf | 2.05 MB | Adobe PDF | OPEN | None | View/Download |
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