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Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.xpro.2023.102338
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dc.titleProtocol for identification and validation of IGF2BP1 target genes in pluripotent human embryonic carcinoma cells
dc.contributor.authorMyint, K
dc.contributor.authorChuang, LSH
dc.contributor.authorMatsuo, J
dc.contributor.authorIto, Y
dc.date.accessioned2024-06-11T07:09:49Z
dc.date.available2024-06-11T07:09:49Z
dc.date.issued2023-06-16
dc.identifier.citationMyint, K, Chuang, LSH, Matsuo, J, Ito, Y (2023-06-16). Protocol for identification and validation of IGF2BP1 target genes in pluripotent human embryonic carcinoma cells. STAR Protocols 4 (2) : 102338-. ScholarBank@NUS Repository. https://doi.org/10.1016/j.xpro.2023.102338
dc.identifier.issn2666-1667
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/248782
dc.description.abstractWe present a detailed protocol to identify and validate IGF2BP1 target genes in pluripotent human embryonic carcinoma cells (NTERA-2). We first identify the target genes through RNA-immunoprecipitation (RIP) sequencing. We then validate the identified targets through the use of RIP-qPCR assays, determine the m6A status of target genes by m6A-IP, and perform functional validation by quantifying changes in mRNA or protein expression levels upon knockdown of IGF2BP1 or methyltransferases in NTERA-2. For complete details on the use and execution of this protocol, please refer to Myint et al. (2022).1
dc.publisherElsevier BV
dc.sourceElements
dc.subjectCancer
dc.subjectCell Biology
dc.subjectCell-based Assays
dc.subjectMolecular Biology
dc.typeArticle
dc.date.updated2024-06-11T02:01:50Z
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentMEDICINE
dc.description.doi10.1016/j.xpro.2023.102338
dc.description.sourcetitleSTAR Protocols
dc.description.volume4
dc.description.issue2
dc.description.page102338-
dc.published.statePublished
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