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https://doi.org/10.1021/bm5001907
Title: | Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells | Authors: | Kandasamy, Karthikeyan Narayanan, Karthikeyan Ni, Ming Du, Chan Wan, Andrew CA Zink, Daniele |
Keywords: | Science & Technology Life Sciences & Biomedicine Physical Sciences Biochemistry & Molecular Biology Chemistry, Organic Polymer Science Chemistry SELF-RENEWAL BIOARTIFICIAL KIDNEYS SURFACE MODIFICATION COATINGS DERIVATION GROWTH DIFFERENTIATION BIOMATERIALS PERFORMANCE PROSPECTS |
Issue Date: | Jun-2014 | Publisher: | AMER CHEMICAL SOC | Citation: | Kandasamy, Karthikeyan, Narayanan, Karthikeyan, Ni, Ming, Du, Chan, Wan, Andrew CA, Zink, Daniele (2014-06). Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells. BIOMACROMOLECULES 15 (6) : 2067-2078. ScholarBank@NUS Repository. https://doi.org/10.1021/bm5001907 | Abstract: | Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-l-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required. Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential. © 2014 American Chemical Society. | Source Title: | BIOMACROMOLECULES | URI: | https://scholarbank.nus.edu.sg/handle/10635/245753 | ISSN: | 1525-7797 1526-4602 |
DOI: | 10.1021/bm5001907 |
Appears in Collections: | Staff Publications Elements |
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