Please use this identifier to cite or link to this item: https://doi.org/10.1021/bm5001907
Title: Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells
Authors: Kandasamy, Karthikeyan 
Narayanan, Karthikeyan
Ni, Ming
Du, Chan
Wan, Andrew CA 
Zink, Daniele
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Chemistry
SELF-RENEWAL
BIOARTIFICIAL KIDNEYS
SURFACE MODIFICATION
COATINGS
DERIVATION
GROWTH
DIFFERENTIATION
BIOMATERIALS
PERFORMANCE
PROSPECTS
Issue Date: Jun-2014
Publisher: AMER CHEMICAL SOC
Citation: Kandasamy, Karthikeyan, Narayanan, Karthikeyan, Ni, Ming, Du, Chan, Wan, Andrew CA, Zink, Daniele (2014-06). Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells. BIOMACROMOLECULES 15 (6) : 2067-2078. ScholarBank@NUS Repository. https://doi.org/10.1021/bm5001907
Abstract: Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-l-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required. Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential. © 2014 American Chemical Society.
Source Title: BIOMACROMOLECULES
URI: https://scholarbank.nus.edu.sg/handle/10635/245753
ISSN: 1525-7797
1526-4602
DOI: 10.1021/bm5001907
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