Please use this identifier to cite or link to this item: https://doi.org/10.1021/bm5001907
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dc.titlePolysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells
dc.contributor.authorKandasamy, Karthikeyan
dc.contributor.authorNarayanan, Karthikeyan
dc.contributor.authorNi, Ming
dc.contributor.authorDu, Chan
dc.contributor.authorWan, Andrew CA
dc.contributor.authorZink, Daniele
dc.date.accessioned2023-11-06T07:11:03Z
dc.date.available2023-11-06T07:11:03Z
dc.date.issued2014-06
dc.identifier.citationKandasamy, Karthikeyan, Narayanan, Karthikeyan, Ni, Ming, Du, Chan, Wan, Andrew CA, Zink, Daniele (2014-06). Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells. BIOMACROMOLECULES 15 (6) : 2067-2078. ScholarBank@NUS Repository. https://doi.org/10.1021/bm5001907
dc.identifier.issn1525-7797
dc.identifier.issn1526-4602
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/245753
dc.description.abstractClinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-l-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required. Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential. © 2014 American Chemical Society.
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysical Sciences
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry, Organic
dc.subjectPolymer Science
dc.subjectChemistry
dc.subjectSELF-RENEWAL
dc.subjectBIOARTIFICIAL KIDNEYS
dc.subjectSURFACE MODIFICATION
dc.subjectCOATINGS
dc.subjectDERIVATION
dc.subjectGROWTH
dc.subjectDIFFERENTIATION
dc.subjectBIOMATERIALS
dc.subjectPERFORMANCE
dc.subjectPROSPECTS
dc.typeArticle
dc.date.updated2023-11-06T03:23:59Z
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.1021/bm5001907
dc.description.sourcetitleBIOMACROMOLECULES
dc.description.volume15
dc.description.issue6
dc.description.page2067-2078
dc.published.statePublished
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