Please use this identifier to cite or link to this item:
https://doi.org/10.1021/bm5001907
DC Field | Value | |
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dc.title | Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells | |
dc.contributor.author | Kandasamy, Karthikeyan | |
dc.contributor.author | Narayanan, Karthikeyan | |
dc.contributor.author | Ni, Ming | |
dc.contributor.author | Du, Chan | |
dc.contributor.author | Wan, Andrew CA | |
dc.contributor.author | Zink, Daniele | |
dc.date.accessioned | 2023-11-06T07:11:03Z | |
dc.date.available | 2023-11-06T07:11:03Z | |
dc.date.issued | 2014-06 | |
dc.identifier.citation | Kandasamy, Karthikeyan, Narayanan, Karthikeyan, Ni, Ming, Du, Chan, Wan, Andrew CA, Zink, Daniele (2014-06). Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells. BIOMACROMOLECULES 15 (6) : 2067-2078. ScholarBank@NUS Repository. https://doi.org/10.1021/bm5001907 | |
dc.identifier.issn | 1525-7797 | |
dc.identifier.issn | 1526-4602 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/245753 | |
dc.description.abstract | Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-l-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required. Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential. © 2014 American Chemical Society. | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Physical Sciences | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Chemistry, Organic | |
dc.subject | Polymer Science | |
dc.subject | Chemistry | |
dc.subject | SELF-RENEWAL | |
dc.subject | BIOARTIFICIAL KIDNEYS | |
dc.subject | SURFACE MODIFICATION | |
dc.subject | COATINGS | |
dc.subject | DERIVATION | |
dc.subject | GROWTH | |
dc.subject | DIFFERENTIATION | |
dc.subject | BIOMATERIALS | |
dc.subject | PERFORMANCE | |
dc.subject | PROSPECTS | |
dc.type | Article | |
dc.date.updated | 2023-11-06T03:23:59Z | |
dc.contributor.department | CHEMISTRY | |
dc.contributor.department | OBSTETRICS & GYNAECOLOGY | |
dc.description.doi | 10.1021/bm5001907 | |
dc.description.sourcetitle | BIOMACROMOLECULES | |
dc.description.volume | 15 | |
dc.description.issue | 6 | |
dc.description.page | 2067-2078 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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File | Description | Size | Format | Access Settings | Version | |
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Kandasamy et al_Biomacromolecules 2014.pdf | 2.21 MB | Adobe PDF | CLOSED | Published |
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